Sequences from confirmation amplicon


Confirmation sequences for line 480C07 and hit At1g49240 are shown.
Line ID 480C07
Locus At1g49240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18216543
Primers used for PCR KH06 - 8474, show primer details
   
Sequence Name 41-K025675-022-480-C07C-8409
GenBank Accession LN503937 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K025675-022-480-C07C-8409
TAGATTTCCCGGACATGAAGCCATGTAGATGTCCGATGCTGATGACATTCAACCTATTGT
CTGTGACAATGGTACTGGAATGGTTAAGGTTAGTCTCTTCTTTCTTACTTTAAGGATTCT
CTTAATTTTGGCAAGATTTGATACAAGTTCCTTTGTGCAGGCTGGATTCGCTGGAGATGA
TGCTCCCAGAGCGGTTTTCCCCAGTGTTGTTGGTCGACCTAGACATCATGGTGTCATGGT
TGGGATGAATCAGAAAGATGCGTATGTTGGTGATGAAGCACAATCCAAAAGAGGTATCCT
CACATTGAAATACCCTATTGAGCATGGGTG
   
Sequence Name 42-K025675-022-480-C07C-KH06
GenBank Accession LN504324 [GenBank]
Sequencing primer gene specific primer, KH06: 5'-ACACCATGCTCAATAGGGTATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K025675-022-480-C07C-KH06
CCTCTTTTGGATTGTGCTTCATCACCAACATACGCATCTTTCTGATTCATCCCAACCATG
ACACCATGATGTCTAGGTCGACCAACAACACTGGGGAAAACCGCTCTGGGAGCATCATCT
CCAGCGAATCCAGCCTGCACAAAGGAACTTGTATCAAATCTTGCCAAAATTAAGAGAATC
CTTAAAGTAAGAAAGAAGAGACTAACCTTAACCATTCCAGTACCATTGTCACAGACAATA
GGTTGAATGTCATCAGCATCGGACATCTACATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCACGT

Last Updated on 06.12.2018 16:11