Sequences from confirmation amplicon


Confirmation sequences for line 480D12 and hit At4g18940 are shown.
Line ID 480D12
Locus At4g18940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10373515
Primers used for PCR 21xp - 8474, show primer details
   
Sequence Name 57-K119542-0022-480-D12C-21xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21xp: 5'-TGAAGGTTTTAAGGTGTATCCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K119542-0022-480-D12C-21xp
ACTTCTACTTTCGTGTCTCTACGTATCTCTCCGGCACACCGTAGAGGTATATTTTACATG
CATAAACTGAAACAAATCTTTAACCGAAATTGAAACTTATAAGCTACCGGACATTGTTTT
TGCGTTTGTCTTATGTTTACAGGTAATGAATGCTGCCGGACATTTCATGGCCCACTTCAA
ACCCTTCACCGGAAAATGTATGATCTAATCTCGATTAATCGGATTTTAATATTTGGTTGG
GAAGTAATTAAAACGTTATTATTGTGAAAAACACTAAAAATCATATTATTACCAAGAAAC
GTGTTTTTTTGTTTCTTTCTCTTTCTGTGTACGTGTATTTGAAACGTTGGATTGTTGCAG
TTTACGTGCCACACATGAGCATACTCTACGGCGATTTAACGGAGAGGGAGAAGAAGAAAG
CGTTAGAGAAAGCTTACACGCTTGATTCGAGTCTCGACGGATTGAATTTTAAGATAAATC
GAGTTGCAATCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 25-K119541-0022-480-D12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119541-0022-480-D12C-8409
CATTTACTGTAGATTGCAACTCGATTTATCTTAAAATTCAATCCGTCGAGACTCGAATCA
AGCGTGTAAGCTTTCTCTAACGCTTTCTTCTTCTCCCTCTCCGTTAAATCGCCGTAGAGT
ATGCTCATGTGTGGCACGTAAACTGCAACAATCCAACGTTTCAAATACACGTACACAGAA
AGAGAAAGAAACAAAAAAACACGTTTCTTGGTAATAATATGATTTTTAGTGTTTTTCACA
ATAATAACGTTTTAATTACTTCCCAACCAAATATTAAAATCCGATTAATCGAGATTAGAT
CATACATTTTCCGGTGAAGGGTTTGAAGTGGGCCATGAAATGTCCGGCAGCATTCATTAC
CTGTAAACATAAGACAAACGCAAAAACAATGTCCGGTAGCTTATAAGTTTCAATTTCGGT
TAAAGATTTGTTTCAGTTTATGCATGTAAAATATACCTCTACGGTGTGCCGGAGAGATAC
GTAGAGACACTGAAAGTAAGAAGTTCCGGCGGAGACTTGGTCAACGGTAGCAGGATACAC
CTTANAACCTTC

Last Updated on 06.12.2018 16:11