Sequences from confirmation amplicon


Confirmation sequences for line 481G09 and hit At1g21000 are shown.
Line ID 481G09
Locus At1g21000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7338731
Primers used for PCR 17so - 8474, show primer details
   
Sequence Name 55-K116453-0022-481-G09C-17so
GenBank Accession LN508915 [GenBank]
Sequencing primer gene specific primer, 17so: 5'-CTTGATTCTTTCAGGTTCTGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K116453-0022-481-G09C-17so
TTTTTCCCTGTTTTACCATTTTTGTTTGAAACAATTCATATGGTTTTAACAAAAATGTAA
ACTTTCTTAAAACCCATTAACGCATTTCACTTCTTAATGATATCTATATCAAATTTTATC
CGGTTTTGTTTAGTTTTGTTCGTGATTCATGTGGATATGCTCTGTTTTTGTAATGTTATT
TGTGGAAAACATGGCTTCATGGAAATATATGGCTCCATATTGGCCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGGTTATTAT
   
Sequence Name 22-K116452-0022-481-G09C-8409
GenBank Accession LN496269 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116452-0022-481-G09C-8409
ATTACATTGAATATATCCTGGCCAATATGGAGCCATATATTTCCATGAAGCCATGTTTTC
CACAAATAACATTACAAAAACAGAGCATATCCACATGAATCACGAACAAAACTAAACAAA
ACCGGATAAAATTTGATATAGATATCATTAAGAAGTGAAATGCGTTAATGGGTTTTAAGA
AAGTTTACATTTTTGTTAAAACCATATGAATTGTTTCAAACAAAAATGGTGAAAACAGGG
GAAAAAACAGAGGGAGAGTCTTTTTGTGTTATACCTTGCAGCCGAGAGAGCAGAACCTGA
AAGAATCAAG

Last Updated on 06.12.2018 16:11