Sequences from confirmation amplicon


Confirmation sequences for line 481H08 and hit At3g52890 are shown.
Line ID 481H08
Locus At3g52890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19611703
Primers used for PCR 44sm - 8474, show primer details
   
Sequence Name 49-K116569-0022-481-H08C-44sm
GenBank Accession LN506960 [GenBank]
Sequencing primer gene specific primer, 44sm: 5'-AAGGATTTCAGGTCCATACAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116569-0022-481-H08C-44sm
ATCTTGCCGGGTTTCGTGTTTAGCCCTAGGCTCTCCTCAAACCAACAACAAGGTAGAAAA
CCAAAAAGGGGTGATCATCTTTCGAAAACCCAACAACACTTGAGTAGATCATTGCCTCAG
CTCGTGGCTGAACCAACCGAAGCAAGATCCAATTCCTTTGTGGGAACACACGAGTACCTA
GCCCCAGAGATCATCAAAGGAGAAGGACATGGCGCTGCAGTTGACTGGTGGACTTTCGGG
GTTCTCCTTTACGAGCTTTTATACGGGAAAACACCATTCAAAGGTTACAACAACGACGAG
ACTTTGATAAAACGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTTAT
TATAT
   
Sequence Name 20-K116568-0022-481-H08C-8409
GenBank Accession LN495372 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116568-0022-481-H08C-8409
CGTCGTTGTTGTACCTTTGAATGGTGTTTTCCCGTATAAAAGCTCGTAAAGGAGAACCCC
GAAAGTCCACCAGTCAACTGCAGCGCCATGTCCTTCTCCTTTGATGATCTCTGGGGCTAG
GTACTCGTGTGTTCCCACAAAGGAATTGGATCTTGCTTCGGTTGGTTCAGCCACGAGCTG
AGGCAATGATCTACTCAAGTGTTGTTGGGTTTTCGAAAGATGATCACCCCTTTTTGGTTT
TCTACCTTGTTGTTGGTTTGAGGAGAGCCTAGGGCTGAAACACGAAACCTGGCAAGATGG
TTCGGTGATACAGAATGGTTGTATGCAGTTGGATGTGTTGTATGGACTGNAAATCCTT

Last Updated on 06.12.2018 16:11