Sequences from confirmation amplicon


Confirmation sequences for line 482A05 and hit At1g15800 are shown.
Line ID 482A05
Locus At1g15800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5442394
Primers used for PCR 06ve - 8474, show primer details
   
Sequence Name 15-K117874-0022-482-A05C-06ve
GenBank Accession LN493163 [GenBank]
Sequencing primer gene specific primer, 06ve: 5'-TCCTATTTTTGCAATCTAGTGCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117874-0022-482-A05C-06ve
TGGCCAGTTTCACGTCACGATGCTTGGTTCCTGAAACTGTTCGCTGAATTCAAGCCAGTT
AAATTACCCAGTCATCACCGATTCAATGACGAGATTACCCCCGTTCACGCAGTGTGTTTT
AGGGGTAGTTGGGTCAATGAGAGAAGTTATGTCTGGCGTTATTTATAATAGCTTATAATG
AGGGTTGCCTCTTTTATGGGTCGAGAGGCGTTTTCGTGGCGGATTAGGTTTGGGGGTTTG
GTGCGTCTGTGGTGTAATCAGATGCGGCGTGGAACATAATAAGTCTCATAGGTAATAATG
GGCTGACATGGAAAAAAGAAAGTCAAAAACTCTCCGAAGCATCTCTTGGTCAAAATCACT
AACTCCTTTCATATTGGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGGATTTATTA
   
Sequence Name 03-K117873-0022-482-A05C-8409
GenBank Accession LN487668 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117873-0022-482-A05C-8409
ATTACATTGCCAATATGAAAGGAGTTAGTGATTTTGACCAAGAGATGCTTCGGAGAGTTT
TTGACTTTCTTTTTTCCATGTCAGCCCATTATTACCTATGAGACTTATTATGTTCCACGC
CGCATCTGATTACACCACAGACGCACCAAACCCCCAAACCTAATCCGCCACGAAAACGCC
TCTCGACCCATAAAAGAGGCAACCCTCATTATAAGCTATTATAAATAACGCCAGACATAA
CTTCTCTCATTGACCCAACTACCCCTAAAACACACTGCGTGAACGGGGGTAATCTCGTCA
TTGAATCGGTGATGACTGGGTAATTTAACTGGCTTGAATTCAGCGAACAGTTTCAGGAAC
CAAGCATCGTGACGTGAAACTTGGCCACCAACACCAATCACCATCATCTCGATGTGAGGA
GGCACTAGATTGAAAAAATAGGA

Last Updated on 06.12.2018 16:11