Sequences from confirmation amplicon


Confirmation sequences for line 482F02 and hit At2g39110 are shown.
Line ID 482F02
Locus At2g39110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16320544
Primers used for PCR 15fu - 8474, show primer details
   
Sequence Name 57-K111688-0022-482-F02C-15fu
GenBank Accession LN509459 [GenBank]
Sequencing primer gene specific primer, 15fu: 5'-CAATGGTCCCTACAACCTGTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K111688-0022-482-F02C-15fu
CATCAATGGAGACTAACAAGTAAGGCGTAGCGTACCGCGGTGGAAACGTGAGTGATTCCA
TCTGAAGGACCCATTCGAGCCAGTCCGAAATCCGAGAGTTTTGCATTCCAGTTCTCATCA
AGAAGGATATTAGAGGATTTGAAATCCCTAAAGATGATCTGCATATAAGATGAGTATAGT
TTCAAGAACATATGAAAAAGGTTTAAAGGCATTTGTGATCATGGATGGAGAAGATACCTG
AAACTCCATGCCTTGATGAAGATAAGCGAGTCCTCTAGCAGTGTCTTGAGCAATCTTCAG
TCTGGTGGACCAAGGAAGAGGAGTGACTATAAAACGATTGGATAAATGGTCTTGAACACT
TCTGTTTTGTACGTATTCGTAAACAAGTAGCCGTTGGATCCCTCTCTCATCATCCTCAGC
GCAATAACCGATCAGCTTCACCAGATTCGGATGCTCCATTTGTATGTCCGGGAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACNGGTTATT
   
Sequence Name 41-K111684-0022-482-F02C-8409
GenBank Accession LN504124 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K111684-0022-482-F02C-8409
TTCCGGACATACAAATGGAGCATCCGAATCTGGTGAAGCTGATCGGTTATTGCGCTGAGG
ATGATGAGAGAGGGATCCAACGGCTACTTGTTTACGAATACGTACAAAACAGAAGTGTTC
AAGACCATTTATCCAATCGTTTTATAGTCACTCCTCTTCCTTGGTCCACCAGACTGAAGA
TTGCTCAAGACACTGCTAGAGGACTCGCTTATCTTCATCAAGGCATGGAGTTTCAGGTAT
CTTCTCCATCCATGATCACAAATGCCTTTAAACCTTTTTCATATGTTCTTGAAACTATAC
TCATCTTATATGCAGATCATCTTTAGGGATTTCAAATCCTCTAATATCCTTCTTGATGAG
AACTGGAATGCAAAACTCTCGGATTTCGGACTGGCTCGAATGGGTCCTTCAGATGGAATC
ACTCACGTTTCCACCGCGGTACGCTACGCCTTACTTGTTATGTCTCCATTGATTGTTTGA
AACAATTGAAGTCTTACCAAACTATGAATCTACAGGTTGTAGGGACCATT

Last Updated on 06.12.2018 16:11