Sequences from confirmation amplicon


Confirmation sequences for line 484F11 and hit At1g15490 are shown.
Line ID 484F11
Locus At1g15490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5321611
Primers used for PCR LB16 - 8474, show primer details
   
Sequence Name 94-K033717-022-484-F11C-8409
GenBank Accession LN515276 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K033717-022-484-F11C-8409
ATTTACATTGAATAATACTCCATCTGATTACAAGTCTACTCACTTTGATGGGATCGTTTG
TTGCCATCAATCTTTGTGCAGCTTTGAGAGCAAGTAGACCTCCGTCATCATGACCAACAA
ATACTACTGAAGAGAACCCCATCTCATAACAGAAAGCAATAAGCATCTCTACCTGGAAGA
TCCAGGCATTATTTTTGTCAAAGAAAGCACAAATCCCCCATTGAGGTTTAATATCAATAA
TGAGGAGACAACTACCTGATTTTCGAGTGAATAAGGATTGAGCAGCTGTCTTTCTTCCAA
ATCATTCTTATGTGGCCTGGCCGTTAATCCCCAACCAGGCCTATCAAATGCAGTGACAAC
ACAACCAAGCTGCTGAGCCAGTGAACCCATCACATGCCTCCAAGAAAACACTCCTCCTCC
AAATCCATGAACCAGGACCACACCAAACTGACCACTTTCACCCAAGTCTTGCTCCTCAGC
TCCAAAATTGAATACATTCATGTCATCCACTAGTCCCGTTCCTTCCTTAATCTCTTTGAA
AAGAAGAGGAGAAGTAGGAGAACCATC
   
Sequence Name 14-K033717-022-484-F11C-LB16
GenBank Accession LN492408 [GenBank]
Sequencing primer gene specific primer, LB16: 5'-GGATGGTTCTCCTACTTCTCCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K033717-022-484-F11C-LB16
ACGGGACAGTGGATGACATGAATGTATTCAATTTTGGAGCTGAGGAGCAAGACTTGGGTG
AAAGTGGTCAGTTTGGTGTGGTCCTGGTTCATGGATTTGGAGGAGGAGTGTTTTCTTGGA
GGCATGTGATGGGTTCACTGGCTCAGCAGCTTGGTTGTGTTGTCACTGCATTTGATAGGC
CTGGTTGGGGATTAACGGCCAGGCCACATAAGAATGATTTGGAAGAAAGACAGCTGCTCA
ATCCTTATTCACTCGAAAATCAGGTAGTTGTCTCCTCATTATTGATATTAAACCTCAATG
GGGGATTTGTGCTTTCTTTGACAAAAATAATGCCTGGATCTTCCAGGTAGAGATGCTTAT
TGCTTTCTGTTATGAGATGGGGTTCTCTTCAGTAGTATTTGTTGGTCATGATGACGGAGG
TCTACTTGCTCTCAAAGCTGCACAAAGATTGATGGCAACAAACGATCCCATCAAAGTGAG
TAGACTTGTAATCAGATGGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11