Sequences from confirmation amplicon


Confirmation sequences for line 485C03 and hit At5g60300 are shown.
Line ID 485C03
Locus At5g60300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24266584
Primers used for PCR EQ79 - 8474, show primer details
   
Sequence Name 38-K103355-0022-485-C03C-EQ79
GenBank Accession LN502944 [GenBank]
Sequencing primer gene specific primer, EQ79: 5'-GGTATTGCAGAAGAAAACGAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103355-0022-485-C03C-EQ79
TGCCCATGGTAGTCTTGACGAGCATTTGTTTGATGACCAGAAACCGGTTCTTTCTTGGTC
ACAGAGACTTGTGGTCGTTAAGGGTATAGCCTCTGCTCTCTGGTACCTGCATACAGGTGC
TGACCAAGTTGTTCTGCACCGAGATGTCAAAGCTTCCAACATTATGTTAGACGCGGAGTT
CCATGGAAGGTTAGGGGATTTTGGCATGGCCAGGTTTCATGAGCACGGAGGCAATGCAGC
TACAACAGCAGCTGTTGGGACTGTAGGGTACATGGCCCCGGAGCTAATCACAATGGGAGC
TTCCACTGGAACTGATGTTTACGCATTTGGTGTTTTCATGCTTGAAGTAACCTGTGGGAG
GAGACCCGTGGAACCGCAGTTGCAAGTTGAGAAACGACATATGATCAAATGGGTTTGTGA
GTGCTGGAAAAAGGATTCTTTGCTTGATGCTACTATGATCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGACCGCA
   
Sequence Name 54-K103356-0022-485-C03C-8409
GenBank Accession LN508447 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103356-0022-485-C03C-8409
GCCATTTACAATTGATCATAGTAGCATCAAGCAAAGAATCCTTTTTCCAGCACTCACAAA
CCCATTTGATCATATGTCGTTTCTCAACTTGCAACTGCGGTTCCACGGGTCTCCTCCCAC
AGGTTACTTCAAGCATGAAAACACCAAATGCGTAAACATCAGTTCCAGTGGAAGCTCCCA
TTGTGATTAGCTCCGGGGCCATGTACCCTACAGTCCCAACAGCTGCTGTTGTAGCTGCAT
TGCCTCCGTGCTCATGAAACCTGGCCATGCCAAAATCCCCTAACCTTCCATGGAACTCCG
CGTCTAACATAATGTTGGAAGCTTTGACATCTCGGTGCAGAACAACTTGGTCAGCACCTG
TATGCAGGTACCAGAGAGCAGAGGCTATACCCTTAACGACCACAAGTCTCTGTGACCAAG
AAAGAACCGGTTTCTGGTCATCAAACAAATGCTCGTCAAGACTACCATTGGGCATGTACT
CTGAGACAAGAAGAAGTTCTCGTTTTCTTCTGNCAATAC
Primers used for PCR KU54 - 8474, show primer details
   
Sequence Name 58-K118780-0022-485-C03C-KU54
GenBank Accession LN509839 [GenBank]
Sequencing primer gene specific primer, KU54: 5'-CTTCTCTGGTTCTGCAACTATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118780-0022-485-C03C-KU54
CTGATCTGTTGATGCCAAGCGTAAGGTGAGTGGTTGGGCGAGGATGAAGACATCGATGGA
CCTGACAAACTCCATGAAGCGGAAGAGACTACAAGGGAGGATGCATCTACCAGAACCGGA
GCAAATGTGCCAATCCCCAAAGTGTATGGTGAGAAATCTGGCAAAGGTAGGTTTTTGTTT
AGGTACAGGACCACTTGTTCCATTGTGGGTCTGGATTCTGGCACTATATTTGAACAGAGC
AGACCCAGTTTCATAACCATCTCGACTTCCTCAGCTACAAATTTACCTCCCAAACCCATG
GATCAATTTACATGCAAATGGGTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTACGCACGGTTATTA
   
Sequence Name 18-K118779-0022-485-C03C-8409
GenBank Accession LN494525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118779-0022-485-C03C-8409
AACCCATTTGCATGTAAATTGATCCATGGGGTTTGGGAGGTAAATTTGTAGCTGAGGAAG
TCGAGATGGTTATGAAACTGGGTCTGCTCTGTTCAAATATAGTGCCAGAATCCAGACCCA
CAATGGAACAAGTGGTCCTGTACCTAAACAAAAACCTACCTTTGCCAGATTTCTCACCAT
ACACTTTGGGGATTGGCACATTTGCTCCGGTTCTGGTAGATGCATCCTCCCTTGTAGTCT
CTTCCGCTTCATGGAGTTTGTCAGGTCCATCGATGTCTTCATCCTCGCCCAACCACTCAC
CTTACGCTTGGCAGTCAACAGATCAGCCATGGGGACAGACAATAGACACTAAGAACTCTC
TTCACATAGTTGCAGACCCAGAGAAG

Last Updated on 06.12.2018 16:11