Sequences from confirmation amplicon


Confirmation sequences for line 485F03 and hit At3g59350 are shown.
Line ID 485F03
Locus At3g59350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21933963
Primers used for PCR TN12 - 8474, show primer details
   
Sequence Name 01-K103793-0022-485-F03C-TN12
GenBank Accession LN486545 [GenBank]
Sequencing primer gene specific primer, TN12: 5'-GTACTCCTCTTTGTGATTTCCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103793-0022-485-F03C-TN12
GATCTTGTTGTTGCAAATTCATTTTGTTTCGGTTTTTGTTTTGTTGTATTGAAGGGCATC
ACACTAGAAAACCACAGGCGGCTGTGAAGCCTGATGCTTTGAAGGAGCCTCCATCTATTG
ATGTGCCTGCATTGTCATTGGATGAGTTGAAAGAAAAGACCGATAACTTTGGATCAAAGT
CATTGATTGGTGAAGGATCTTACGGCAGAGCCTATTATGCAACCTTGAAAGATGGAAAGG
CTGTCGCGGTGAAGAAGCTTGACAATGCAGCGGAACCTGAATCAAATGTTGAGTTCTTGA
CTCAGGTCTCTAGGGTTTCCAAGCTGAAGCACGATAATTTTGTTGAGCTCTTCGGTTATT
GTGTTGAAGGGAATTTCCGCATTCTTGCGTATGAGTTTGCTACTATGGGATCTTTACAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATAT
AAATATCCGCAGCGT
   
Sequence Name 09-K103794-0022-485-F03C-8409
GenBank Accession LN490146 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103794-0022-485-F03C-8409
GCCATTTACATTGAATAATCCTGTAAAGATCCCATAGTAGCAAACTCATACGCAAGAATG
CGGAAATTCCCTTCAACACAATAACCGAAGAGCTCAACAAAATTATCGTGCTTCAGCTTG
GAAACCCTAGAGACCTGAGTCAAGAACTCAACATTTGATTCAGGTTCCGCTGCATTGTCA
AGCTTCTTCACCGCGACAGCCTTTCCATCTTTCAAGGTTGCATAATAGGCTCTGCCGTAA
GATCCTTCACCAATCAATGACTTTGATCCAAAGTTATCGGTCTTTTCTTTCAACTCATCC
AATGACAATGCAGGCACATCAATAGATGGAGGCTCCTTCAAAGCATCAGGCTTCACAGCC
GCCTGTGGTTTTCTAGTGTGATGCCCTTCAATACAACAAAACAAAAACCGAAACAAAATG
AATTTGCATACAACAAGATCTGTTACTAGCGCAAGAAATGATCAGCTCAAGGGCAGGAAA
TCACAAAGAGGAGTA

Last Updated on 06.12.2018 16:11