Sequences from confirmation amplicon


Confirmation sequences for line 488B12 and hit At2g39660 are shown.
Line ID 488B12
Locus At2g39660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16533602
Primers used for PCR 46nb - 8474, show primer details
   
Sequence Name 25-K114626-0022-488-B12C-46nb
GenBank Accession LN497561 [GenBank]
Sequencing primer gene specific primer, 46nb: 5'-AAATATGAATTATGACTAGGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114626-0022-488-B12C-46nb
GATATTGTTTTTAATCCTCGTATCATTTCAGGTCACTTGAATGCAAGAAGCGATGTGTAC
AGTTTCGGAGTTTTGCTTCTAGAGATTTTATCTGGTAAGCGAGCGTTGGATCATAACAGA
CCGGCTAAAGAAGAAAACCTTGTGGATTGGGCTCGACCGTACCTCACAAGCAAACGTAAG
GTTCTCCTAATCGTGGACAATCGGCTAGACACACAGTACCTACCTGAAGAAGCCGTGAGA
ATGGCAAGCGTGGCGGTGCAGTGTCTCTCATTTGAACCCAAGTCGCGCCCGACCATGGAC
CAAGTGGTCCGTGCCTTACAACAACTTCAGGACAACTTGGGAAAACCGAGTCAGACCAAT
CCGGTTAAGGATACCAAGAAACTTGGTTTTAAAACTGGTACTACTAAGTCATCCGAAAAA
CGGTTTACACAAAAACCTTTTGGCAGGCACCTTGTGTAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 25-K114625-0022-488-B12C-8409
GenBank Accession LN497560 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114625-0022-488-B12C-8409
GCCTTTCATTGAATTATCCTACACAAGGTGCCTGCCAAAGGTTTTTGTGTAAACCGTTTT
TCGGATGACTTAGTAGTACCAGTTTTAAAACCAAGTTTCTTGGTATCCTTAACCGGATTG
GTCTGACTCGGTTTTCCCAAGTTGTCCTGAAGTTGTTGTAAGGCACGGACCACTTGGTCC
ATGGTCGGGCGCGACTTGGGTTCAAATGAGAGACACTGCACCGCCACGCTTGCCATTCTC
ACGGCTTCTTCAGGTAGGTACTGTGTGTCTAGCCGATTGTCCACGATTAGGAGAACCTTA
CGTTTGCTTGTGAGGTACGGTCGAGCCCAATCCACAAGGTTTTCTTCTTTAGCCGGTCTG
TTATGATCCAACGCTCGCTTACCAGATAAAATCTCTAGAAGCAAAACTCCGAAACTGTAC
ACATCGCTTCTTGCATTCAAGTGACCTGAAATGATACGAGGATTTAAAAACAATTATCCT
TATGCTCTAAAACATTTGATCAACCTAGTCATAATTCATATT

Last Updated on 06.12.2018 16:11