Sequences from confirmation amplicon


Confirmation sequences for line 488D10 and hit At5g54430 are shown.
Line ID 488D10
Locus At5g54430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22098964
Primers used for PCR HF44 - 8474, show primer details
   
Sequence Name 48-K101326-0022-488-D10C-8409
GenBank Accession LN506456 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101326-0022-488-D10C-8409
TTCGGACTGAGCCTTTACAACGGAAGGTCTATGTGATTCAGTGATTGATTAGATAAGCTG
CTTTCGTTGTTATTTTTTTCATGGATGTTTCTGTTGATGTGTCAGATTTAGATTCAGTAG
TGTAGTGAGTGTTGTGAATTTTGTGATCACGTGAATATGTTGTGAAAAAGATTGCCATAG
ATGGTAGCACAGGAAAATGAGATCCTTTTATAGCTGAACTAAGATGATTTTGTCGATTTA
GTGAGAAATGCTGTCCATGTTCATTGCCTTGCTTACCCGAAAATTGAGGATGATGGTCAT
GTACATAGATGATTTCCTAGCTTTAGTGTATTCGTTCTATGAATTTGTATATATCTTCTT
CATCTGCCGAAACTGTTTCTATTTACTGAAGTTAGTTGTATAGTATATATCATAATTTTT
GGTGGTTATGGCTAATTTGCTGTAATATATGTTAGAAGATTATTGTTCCCCTACACATGG
GACAAAGAAGCGCCGACATCGTTCTATTTCTTGCATTGTTTACACTACCCATTGATGGCG
TT
   
Sequence Name 48-K101332-0022-488-D10C-HF44
GenBank Accession LN506457 [GenBank]
Sequencing primer gene specific primer, HF44: 5'-TAAACGCAATCAATGGGTAGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101332-0022-488-D10C-HF44
CGGCGCTTCTTTGTCCATGTGTAGGGGAACATAATCTTCTAACNACCCTTCAGCAAATTA
GCCATAACCACCAAAAATTATGATATCTACTATACAACTAACTTCAGTAAATAGAAACAG
TTTCGGCAGATGAAGAAGATATATACAAATTCATAGAACGAATACACTAAAGCTAGGAAA
TCATCTATGTACATGACCATCATCCTCAATTTTCGGGTAAGCAAGGCAATGAACATGGAC
AGCATTTCTCACTAAATCGACAAAATCATCTTAGTTCAGCTATAAAAGGATCTCATTTTC
CTGTGCTACCATCTATGGCAATCTTTTTCACAACATATTCACGTGATCACAAAATTCACA
ACACTCACTACACTACTGAATCTAAATCTGACACATCAACAGAAACATCCATGAAAAAAA
TAACAACGAANGCAGCTTATCTAATCAATCACTGAATCACATAGACCTTTCCGTTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR HF45 - 8474, show primer details
   
Sequence Name 49-K101327-0022-488-D10C-8409
GenBank Accession LN506774 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101327-0022-488-D10C-8409
CGGACATGAGCCTTTACAATTGAATTACAACGAAAGCAGCTTATCCCTGACCTTCGATGT
GTTCATGATGAGCAGAAGCAGAAGCAGCAACAACATCTCCATCATCATCTCCACCAGACT
TGACAGTAACAATAGGCACAGGTCCATCACGATCATCAGGATATCTAACCACAACAACAG
GACAAACACAGTGATGAACACAGTAATCACTAACAGAGCCAAGCTTGCCATCACTTCCTC
TTTTTTTCTCAGCACCAAAACCTCTACTTCCCATAATCACAGCACTAAGACCAAGCCTCT
CAATCTCAAGACATAATCTCTCTCTCATATCATGATCTTTCACTATATGGATCTTATAAG
GAAACCCTAACTCCTTCAACGGTTTAGCTAGATCCGCAACTTTTGTTGAAGTAAAAGCAT
CAAAATCCTCTTGACTAGGNTGAGGTTGAGA
   
Sequence Name 49-K101333-0022-488-D10C-HF45
GenBank Accession LN506775 [GenBank]
Sequencing primer gene specific primer, HF45: 5'-CTCAACCTCAACCTAGTCAAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101333-0022-488-D10C-HF45
TACAAAGTTGCGGATCTGCTAACCGTTGAAGGAGTTAGGGTTTCCTTATAACATTATATA
GTGAAAGATCATGATATGAGAGAGAGATTATCTCTTGAGATTGAGAGGCTTGGTCTTAGT
GCTGTGATTATGGGAAGTAGAGGTTTTGGTGCTGAGAAAAAAAGAGGAAGTGATGGCAAG
CTTGGCTCTGTTAGTGATTACTGTGTTCATCACTGTGTTTGTCCTGTTGTTGTGGTTAGA
TATCCTGATGATCGTGATGGACCTGTGCCTATTGTTACTGTCAAGTCTGGTGGAGATGAT
GATGGAGATGTTGTTGCTGCTTCTGCTTCTGCTCATCATGAACACATCANAGGTCAGGGA
TAAGCTGCTTTCGTTGTAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCANAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11