Sequences from confirmation amplicon


Confirmation sequences for line 490C10 and hit At5g39300 are shown.
Line ID 490C10
Locus At5g39300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15738130
Primers used for PCR NB90 - 8474, show primer details
   
Sequence Name 05-K034734-022-490-C10C-8409
GenBank Accession LN488255 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K034734-022-490-C10C-8409
GCCATTTAAATTGAGTTTACATTACTTTTATGTACATTTACAATTGAGTGTACATTACTT
TTGCGATTGCATTTACATATATCTTAATGTTTTGATCAAAGAAAAAAATGTACATAAAAT
TAATGTTTATTTATTTTTATTCAATATAGAGGGAGCCTGTGGATACGGTGATTTATTCAA
ACAAGGCTATGGTCTAGAGACAGCGGCATTGAGCACAGCACTCTTCAACGAAGGGTATAC
ATGTGGGGCTTGTTACCAGATCATGTGTGTGCACGACCCACAATGGTGTTTACCCGGAAC
CATCAAGATTACAGCAACAAATTTCTGTC
   
Sequence Name 06-K034734-022-490-C10C-NB90
GenBank Accession LN488707 [GenBank]
Sequencing primer gene specific primer, NB90: 5'-GTGGACAGAAATTTGTTGCTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K034734-022-490-C10C-NB90
GTGGGTCGTGCACACACATGATCTGGTAACAAGCCCCACATGTATACCCTTCGTTGAAGA
GTGCTGTGCTCAATGCCGCTGTCTCTAGACCATAGCCTTGTTTGAATAAATCACCGTATC
CACAGGCTCCCTCTATATTGAATAAAAATAAATAAACATTAATTTTATGTACATTTTTTT
CTTTGATCAAAACATTAAGATATATGTAAATGCAATCGCAAAAGTAATGTACACTCAATT
GTAAATGTACATAAAAGTAATGTAAACTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCATCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11