Sequences from confirmation amplicon


Confirmation sequences for line 491C10 and hit At5g40170 are shown.
Line ID 491C10
Locus At5g40170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16067169
Primers used for PCR PG22 - 8474, show primer details
   
Sequence Name 49-K101746-0022-491-C10C-8409
GenBank Accession LN506785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101746-0022-491-C10C-8409
CATTTACAATTGAATATATCCTGAAAATGGCTATACCCTCTGGATTTGGTAGACTGACCT
ACTTGGAATCCTTGGATCTTAGCAAAAATGGGTTTATAGGAGAAGTTCCATCCTCAATAA
GTAACCTAAGCCGGTTAACCAATTTAGACCTTTCATATAACAAGCTCACCGGTGGTATTC
CCAATCTACATAGTTTAACCCTTCTTGAGAATATAGACCTATCATATAATAAGTTTTCTG
GAGCCATTCCTTCTTACCTCTTCACCATGCCTTTTCTAGTTTCTCTTAACCTACGCCAAA
ACCATCTTAGTGACCCTCTTGAGAATATAAATTATTCTGCAACCTCTAAACTTTTAATTT
TAGACATGGCTTATAACCTTATGAGTCATCGAATCCTAGAACCTATCTCAAAATTAGCCA
ACCTCATACAAATTGACTTATCTTTTCAGAAGACACCCTACACATTCAACTTTGATTTCT
TGCTCTTCAAGTCTCTAGTGCGTTTGGATCTTTCCGGAAATAGTGTTTCAGTGGTTGGTA
CCGGTTCAGAAAATTTGACACATCTNGACTTGTCGAGCTGCAACATCACCGAGTTCCCGA
TGTTTATTAAGGACTTACAAAGATTATGGTGGTTAGACATTTCCNATAATAGAATTAAAG
GAAAAGTGCCTGAGTTGTTATGGACTCTGCCTTCTATGCTCCAT
   
Sequence Name 49-K101752-0022-491-C10C-PG22
GenBank Accession LN506786 [GenBank]
Sequencing primer gene specific primer, PG22: 5'-CGTGAGAGATTTACATGGAGCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101752-0022-491-C10C-PG22
CTCGGCACTTTTCCTTTATTCTATTATTGGAAATGTCTAACCACCATAATCTTTGTAAGT
CCTTAATAAACATCGGGAACTCGGTGATGTTGCAGCTCGACAAGTCTAGATGTGTCAAAT
TTTCTGAACCGGTACCAACCACTGAAACACTATTTCCGGAAAGATCCAAACGCACTAGAG
ACTTGAAGAGCAAGAAATCAAAGTTGAATGTGTAGGGTGTCTTCTGAAAAGATAAGTCAA
TTTGTATGAGGTTGGCTAATTTTGAGATAGGTTCTAGGATTCGATGACTCATAAGGTTAT
AAGCCATGTCTAAAATTAAAAGTTTAGAGGTTGCAGAATAATTTATATTCTCAAGAGGGT
CACTAAGATGGTTTTGGCGTAGGTTAAGAGAAACTAGAAAAGGCATGGTGAAGAGGTAAG
AAGGAATGGCTCCNGAAAACTTATTATATGATAGGTCTATATTCTCAAGAAGGGTTAAAC
TATGTAGATTGGGAATACCACCGGTGAGCTTGTTATATGAAAGGTCTAAATTGGTTAACC
GGCTTAGGTTACTTATTGAGGATGGAACTTCTCCTATAAACCCATTTTTGCTAAGATCCA
AGGATTCCAAGTAGGTCAGTCTACCAAATCCAGAGGGTATAGCCNTTTTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTA

Last Updated on 06.12.2018 16:11