Sequences from confirmation amplicon


Confirmation sequences for line 492B08 and hit At5g15950 are shown.
Line ID 492B08
Locus At5g15950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5207334
Primers used for PCR NU40 - 8474, show primer details
   
Sequence Name 14-K103931w-0022-492-B08C-NU40
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NU40: 5'-TGAGTGCACTATCGTCTCATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103931w-0022-492-B08C-NU40
GTTCTCTCTGAGTCCGTCTCTTTATCTTCCCGTACAAGATCGTCATCAAGACTTGCGGTA
CTACTAAGCTCCTCCTCTCTATTGAGCCTCTCTTGAGGTTAGCCGGTGAGCTTTCTCTTG
ACGTTAAGGCTGTGAGGTACACTCGTGGAAGTTTCCTCTGTCCTGGAGGACAACCATTCC
CTCACCGCAACTTCTCTGAAGAAGTCTCTGTTCTTGATGGTCACTTTGCTAAGCTTGGTT
TAAGCAGTGTTGCCTACTTGATGGGCAATGACGATGAAACCAAGAAATGGCATGTTTACT
CTGCTTCTTCTGCCAACAGCAACAACAAGAACAATGTCTACACGCTTGAGATGTGTATGA
CTGGTCTAGACAAAGACAAGGCATCTGTCTTCTACAAGAACGAGTCAAGCTCTGCTGGTT
CTATGACTGATAACTCTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTAT
   
Sequence Name 30-K103932w-0022-492-B08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103932w-0022-492-B08C-8409
CGGACTGAGCCGAGTTATCAGTCATAGAACCAGCAGAGCTTGACTCGTTCTTGTAGAAGA
CAGATGCCTTGTCTTTGTCTAGACCAGTCATACACATCTCAAGCGTGTAGACATTGTTCT
TGTTGTTGCTGTTGGCAGAAGAAGCAGAGTAAACATGCCATTTCTTGGTTTCATCGTCAT
TGCCCATCAAGTAGGCAACACTGCTTAAACCAAGCTTAGCAAAGTGACCATCAAGAACAG
AGACTTCTTCAGAGAAGTTGCGGTGAGGGAATGGTTGTCCTCCAGGACAGAGGAAACTTC
CACGAGTGTACCTCACAGCCTTAACGTCAAGAGAAAGCTCACCGGCTAACCTCAAGAGAG
GCTCAATAGAGAGGAGGAGCTTAGTAGTACCGCAAGTCTTGATGACGATCTTGTACGGGA
AGATAAAGAGACTGGACTCAGAGAGAACATAAGAGTCAAGCTGATCGTTCGAGAGAGATG
AGACGATAGTGCACTC

Last Updated on 06.12.2018 16:11