Sequences from confirmation amplicon


Confirmation sequences for line 492F02 and hit At5g61630 are shown.
Line ID 492F02
Locus At5g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24774253
Primers used for PCR GA77 - 8474, show primer details
   
Sequence Name 42-K101501-0022-492-F02C-8409
GenBank Accession LN504407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101501-0022-492-F02C-8409
CATTTACAATTAGTGAGCCAAAAACATGCTTGTGTTTGATCAAACCATGCCCGGTTTTAA
AGTGAAAGAAAAAACATGCATGTTGTTAACCACGAACTCATCCGCAATAATCCAACATGA
TCAAATTGGATTGCAAATGGTAGAGAGATCATGTCTGCATTTGGCGGTTGTGAAGTTGGG
AAATATTTTATGTAACTGTTACATGTTGATACTTTATTCTTTGTGGCACTTATAACATCG
ATCCAAATTTTGAAGATGTGGATGTTGGGATGAGAGAAGAATCTATTGCGGCTAAGATTG
AAGGATTGACGATCGAAGGAGACATCGACNATGATAGCGATGGAACTCAGACCGGTTTCT
TGGGTGCCACCTTAGAACTCGGAGATCCTGACAAACCAAACCTCACTGATAGAACCAGAC
GAGCTTCTAGGAAATTGATCANAGGATTTTACTAGTTAATCAACACCATTTTTTTAATTT
TCTCCCAATGAATGTTGATTTTACTCAATAAACTTCACCAAAACTAAGATTTTTTTTGTT
TTGTTTTTGGTTGA
   
Sequence Name 42-K101505-0022-492-F02C-GA77
GenBank Accession LN504408 [GenBank]
Sequencing primer gene specific primer, GA77: 5'-GCTGCTTCTTTATCAACCAAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101505-0022-492-F02C-GA77
TGGTGAGTTATTGAGTAAATCACATTCATTGGGAGAAAATTAAAAAAATGGTGTTGATTA
ACTAGTAAAATCCTTTGATCAATTTCCTAGAAGCTCGTCTGGTTCTATCAGTGAGGTTTG
GTTTGTCAGGATCTCCGAGTTCTAAGGTGGCACCCAAGAAACCGGTCTGAGTTCCATCGC
TATCATTGTCGATGTCTCCTTCGATCGTCAATCCTTCAATCTTAGCCGCAATAGATTCTT
CTCTCATCCCAACATCCACATCTTCAAAATTTGGATCGATGTTATAAGTGCCACAAAGAA
TAAAGTATCAACATGTAACAGTTACATAAAATATTTCCCAACTTCACAACCGCCAAATGC
AGACATGATCTCTCTACCATTTGCAATCCAATTTGATCATGTTGGATTATTGCGGATGAG
TTCGTGGTTAACAACATGCATGTTTTTTCTTTCACTTTAAAACCGGGCATGGTTTGATCA
AACACAAGCATGTTTTTGGCTC

Last Updated on 06.12.2018 16:11