Sequences from confirmation amplicon


Confirmation sequences for line 493C05 and hit At2g29890 are shown.
Line ID 493C05
Locus At2g29890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12748457
Primers used for PCR 22of - 8474, show primer details
   
Sequence Name 08-K114782-0022-493-C05C-22of
GenBank Accession LN489842 [GenBank]
Sequencing primer gene specific primer, 22of: 5'-TGGATTGGATCAAACTCAAACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114782-0022-493-C05C-22of
GTATTTTCATCTTTGTTTGGCCTTCCAGTGCTTTTTTTGCTGTTTACGGGTGGAACTTCA
GTAAAAATGGGATCAAAACATCATATGGCATAAATAAATTTTAAGAATGGCGAACTCGGG
GTTACCGAATATGGCTTCCTTTTTCAGTGTTTCTTAGTCCATTGTACTTATGAGATTGCA
GAAATTCCTAGAGATGGATATACTGGAAGAAGGTCTAACCATGAGGACTCCTGTATATGT
TGTCACAGAAGGCCACGAGCCACCATTTTTCACCCGTTTCTTTGAGTGGGTTCCTGAAAA
GGCAAACGTAAGAAATCTTTTCCCATCTNGAAGTCAATTGTAAATGTTTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 08-K114769-0022-493-C05C-8409
GenBank Accession LN489841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114769-0022-493-C05C-8409
ACTTCAGAAGGGAAAAGATTTCTTACGTTTGCCTTTTCAGGAACCCACTCAAAGAAACGG
GTGAAAAATGGTGGCTCGTGGCCTTCTGTGACAACATATACAGGAGTCCTCATGGTTAGA
CCTTCTTCCAGTATATCCATCTCTAGGAATTTCTGCAATCTCATAAGTACAATNGGACTA
AGAAACACTGAAAAAGGAAGCCATATTCGGTAACCCCGAGTTCGCCATTCTTAAAATTTA
TTTATGCCATATGATGTTTTGATCCCATTTTTACTGAAGTTCCACCCGTAAACAGCAAAA
AAAGCACTTGGAAGGCCAAACAAAGATGAAAATTACCAGACCAAGAGTAAGAGCTTCTTC
CTTCGACTTTATGTTTGAG

Last Updated on 06.12.2018 16:11