Sequences from confirmation amplicon


Confirmation sequences for line 493C09 and hit At3g53670 are shown.
Line ID 493C09
Locus At3g53670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19891372
Primers used for PCR 03bh - 8474, show primer details
   
Sequence Name 75-K107432-0022-493-C09C-03bh
GenBank Accession LN513250 [GenBank]
Sequencing primer gene specific primer, 03bh: 5'-CTAGCTCGGAGTAAGCTAATCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K107432-0022-493-C09C-03bh
TCTTTCGGTTTTTGTTTTGACGTTTTGTGTGAACGAGGTAATTTGGCTATATGATTTTTT
GGCTAAAGTGTTGTTTGAGCTAGAGTTCTCTTGTTAATGGTATTTGAATTTGTGTTTGTT
GCAGTTACTTTTGGATATGAGCTCTGTCTGTGGTGGGTTGGATTTCAAAGATGCAGAGAG
CAGCTCTGCTTCCAGTCCCATTATATGTTCTACTGGCGTAAAGCATATCTCTTGTGTTGG
ATCTGATGATGCTCAAGAGTCTGATGGTGATGATAGTGGTTATATACACCAAACTGTGAT
TGAAGAATCCAAAGACAAGGCGATCAGCGAACCCATCCCTGAGTCTTTACCTCTCAATTC
TTTGGATGATGAAAGTGAAGATAAGAACCTTGCTACTGCATTGCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTA
   
Sequence Name 67-K107431-0022-493-C09C-8409
GenBank Accession LN511827 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K107431-0022-493-C09C-8409
GCCATTTACATTGAATATATCCTGCAATGCAGTAGCAAGGTTCTTATCTTCACTTTCATC
ATCCAAAGAATTGAGAGGTAAAGACTCAGGGATGGGTTCGCTGATCGCCTTGTCTTTGGA
TTCTTCAATCACAGTTTGGTGTATATAACCACTATCATCACCATCAGACTCTTGAGCATC
ATCAGATCCAACACAAGAGATATGCTTTACGCCAGTAGAACATATAATGGGACTGGAAGC
AGAGCTGCTCTCTGCATCTTTGAAATCCAACCCACCACAGACAGAGCTCATATCCAAAAG
TAACTGCAACAAACACAAATTCAAATACCATTAACAAGAGAACTCTAGCTCAAACAACAC
TTTAGCCAAAAAATCATATAGCCAAATTACCTCGTTCACACAAAACGTCAAAACAAAAAC
CGAAAGATCGATCAATAACAAGCTTGTTTTCTTCGATTAGCTTACTCCGAGCTA

Last Updated on 06.12.2018 16:11