Sequences from confirmation amplicon


Confirmation sequences for line 493D03 and hit At2g18900 are shown.
Line ID 493D03
Locus At2g18900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8190577
Primers used for PCR PW11 - 8474, show primer details
   
Sequence Name 01-K024846-022-493-D03C-8409
GenBank Accession LN486420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K024846-022-493-D03C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAGCTATATAATCTTCTCAATGACCGGGGAA
TTTCAGAGGTTAGAATTTGGCTCCAATATATCCTTAAGAAACTCTATGCCACTTTCTCCT
GTCTATGAAATCTCTAACTTCTTTAATCAATGCTTTTGCAGATTCAAGTTTGTGAGAGAA
ACCATCAACCAGGAGATGAGATCACAGTAAGTACATTTCCTTGCTGTTTATTTGATAGTA
TATCTAAGATTATTTTCGTTTGGGTGATAATTTGAGTAAACGGTAATAGGTTGTGGTGAC
AGCGGTTGCTCTCTCCCTGGATGGCTCAGTGATGAGTACAACCGAGGTGAAACTTCCGGA
AGATGGAATAGGAGGCTTAGTGTCCCTCAAGTTTTGGGCGTCTGAACCAGACAGCAAAAC
TTTCACCTTATCCACAATCGTATATGAACCTCATAGGTTAATATTTCACCCTCTGCTAAT
GGATAAAGCTTTATTTTTCA
   
Sequence Name 02-K024846-022-493-D03C-PW11
GenBank Accession LN486876 [GenBank]
Sequencing primer gene specific primer, PW11: 5'-TTGGGTAATGGTGAAAAATAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K024846-022-493-D03C-PW11
TCAGAGGGTGAAATATTAACCTATGAGGTTCATATACGATTGTGGATAAGGTGAAAGTTT
TGCTGTCTGGTTCAGACGCCCAAAACTTGAGGGACACTAAGCCTCCTATTCCATCTTCCG
GAAGTTTCACCTCGGTTGTACTCATCACTGAGCCATCCAGGGAGAGAGCAACCGCTGTCA
CCACAACCTATTACCGTTTACTCAAATTATCACCCAAACGAAAATAATCTTAGATATACT
ATCAAATAAACAGCAAGGAAATGTACTTACTGTGATCTCATCTCCTGGTTGATGGTTTCT
CTCACAAACTTGAATCTGCAAAAGCATTGATTAAAGAAGTTAGAGATTTCATAGACAGGA
GAAAGTGGCATAGAGTTTCTTAAGGATATATTGGAGCCAAATTCTAACCTCTGAAATTCC
CCGGTCATTGAGAAGATTATATAGCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGGTCTAC

Last Updated on 06.12.2018 16:11