Sequences from confirmation amplicon


Confirmation sequences for line 494A04 and hit At4g28250 are shown.
Line ID 494A04
Locus At4g28250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14000950
Primers used for PCR JV23 - 8474, show primer details
   
Sequence Name 63-K032928-022-494-A04C-8409
GenBank Accession LN510913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K032928-022-494-A04C-8409
CATTTACAATTGAATATATCCTGGAGTCATGTAGATCATCTACAAGTTTAGTTGATTCAT
TGCTGTTCTTGGCGAATGTGCATGGAGCCAATGTCTCCTTCTCCATCTTCAAACTCGACC
AGCAGAGATAGCCAAAAATCAGTTGATCCTTCGTTCACATGGAATGCTATGTTCTTCCCT
CTATATTTGCATGCAGTCCTTTTTTTATTATGCAAAAGAAAGAAAGAAGGTAAAATTATA
TCAGTGAGAAAAAAGAGATCAAATGGTCAATGGTCAAGAAATGGTTGGGACCATACAAAA
CGAGATCGCCGTTAAAGTTACCTCTTTTGTTTAATACATTCACACCCATTCCAATAAATC
TAATAATTATATTAACTCTGTTTTAGCCTCTTACCTAAATAATTCCTATTTTTCTATATG
CTCGTGCACTGTCACGAGTTAGTTATTGAAAGAGCATAAACATTAATAAATGACAAGAAT
AATGAGAATAATATTAATATACGTACCGGCGATAAATGACTGGGAAAAGTCCAC
   
Sequence Name 63-K032928-022-494-A04C-JV23
GenBank Accession LN510914 [GenBank]
Sequencing primer gene specific primer, JV23: 5'-CGTGGACTAATCCCAGTCATTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K032928-022-494-A04C-JV23
TCTTGTCATTTATTAATGTTTATGCTCTTTCAATAACTAACTCGTGACAGTGCACGAGCA
TATAGAAAAATAGGAATTATTTAGGTAAGAGGCTAAAACAGAGTTAATATAATTATTAGA
TTTATTGGAATGGGTGTGAATGTATTAAACAAAAGAGGTAACTTTAACGGCGATCTCGTT
TTGTATGGTCCCAACCATTTCTTGACCATTGACCATTTGATCTCTTTTTTCTCACTGATA
TAATTTTACCTTCTTTCTTTCTTTTGCATAATAAAAAAAGGACTGCATGCAAATATAGAG
GGAAGAACATAGCATTCCATGTGAACGAAGGATCAACTGATTTTTGGCTATCTCTGCTGG
TCGAGTTTGAAGATGGAGAAGGAGACATTGGCTCCATGCACATTCGCCAAGAACAGCAAT
GAATCAACTAAACTTGTAGATGATCTACATGACTCCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11