Sequences from confirmation amplicon


Confirmation sequences for line 494A05 and hit At5g26220 are shown.
Line ID 494A05
Locus At5g26220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9163718
Primers used for PCR 47by - 8474, show primer details
   
Sequence Name 27-K108417-0022-494-A05C-47by
GenBank Accession LN498422 [GenBank]
Sequencing primer gene specific primer, 47by: 5'-CACTAGTGTGTTGGAATTTTCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K108417-0022-494-A05C-47by
ATTTTCATATCTTTTTTGTATTTTTGGATTAAAATGCACCATTTATTTCCATATAGCACT
GTCCATTTTGCCATATTTAGCTTTAGAAATAGCTTTATACTTAATTAGTCTATGGCAAAA
ATGCAATTTGAAATTGATGTTGAAAATTTCTGTTTAAATTGAGCAGTGGGGTGCCGCTTA
TTGTGTTCCTGGAGGAC
   
Sequence Name 11-K108416-0022-494-A05C-8409
GenBank Accession LN491149 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K108416-0022-494-A05C-8409
ATCATTGTCGTAATTTGTCGTTTTATCAAATAAATAAATAAAACTATTAAAATTCTGGGA
AAAAGACAACAACAATAAAACAGCAACCCGTTTCTTACCGTGTAAAACTCAACAAGTGTT
TTTGAGTCGTATTCACACTCTCTTCGTTCCAAGTACTGCAGAAGAACCCTGAGCCATATC
AACATATATTAAGATCCAAACAAAATAAACACACAAACCCCAAACTAAACCAATAAAAAC
ACCGAATCTTCTTTTTACCTCCATTGCCAGTTTCCCTTTCTCTGGTCCTCCCCCAACC

Last Updated on 06.12.2018 16:11