Sequences from confirmation amplicon


Confirmation sequences for line 494B07 and hit At4g29770 are shown.
Line ID 494B07
Locus At4g29770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14576793
Primers used for PCR GA74 - 8474, show primer details
   
Sequence Name 01-K101423-0022-494-B07C-8409
GenBank Accession LN486509 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K101423-0022-494-B07C-8409
TTTACATTGAAATTGGTACTGACAGCTTGGTTGATCCATTTCTCCCTCAACTTCTTCCTT
CCCAACGTTATCCACCAAATACTGAGGTGAGTACTCAGTATACTTAGAGTCGATCTTGAA
GATAACACGTGCAGAGCTAATCAAACTCACAGCTACCAAAGCTGCAGATTGACATGCATC
TGCAAGACAAAAAGAAAGATAAAAATGTTATAAGTCAACGCATCTGATTATTTCAAAAGG
ATTTAGATTGAACGTGTTAAAACGAGAGTTAATAACAGTTAAAACCAAATCACAGTTTCT
TATAATGATGCAAAGAAAGTAAATTTAAGATTCAAGATTTGATAATATACCGTAATCTTC
TTGCTCATTGTTTGCTTTTAAGCAATCGGGATGACATTTGCCATCCGTGATTGCAAAACA
TTCTAACAATGACTCCATCTGCGATGAACTCCTGGTTTTAAGTAGAAACAAACAAAAGAA
GACATTCACAAATCCAGCACAATCAAGCAGAAGAAAACATTCAGAAACAAGATATCTATT
AAGCAAAACCAAAACAAGACCTAAGTAGCTNAGCATATACACGGTTTCTAGTATTGACAT
AAACAGAACCACCATTTCTAGTTGCAGAAGAAAAA
   
Sequence Name 41-K101426-0022-494-B07C-GA74
GenBank Accession LN504018 [GenBank]
Sequencing primer gene specific primer, GA74: 5'-TTTTCCTCTGCAACTAGAAATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101426-0022-494-B07C-GA74
GCTTAGCTCTTAGGTCTTGTTTTGGTTTTGCTTTATAGATATCTTGTTTCTGAATGTTTT
CTTCTGCTTGATTGTGCTGGATTTGTGAATGTCTTCTTTTGTTTGTTTCTACTTAAAACC
AGGAGTTCATCGCAGATGGAGTCATTGTTAGAATGTTTTGCAATCACGGATGGCAAATGT
CATCCCGATTGCTTAAAAGCAAACAATGAGCAAGAAGATTACGGTATATTATCAAATCTT
GAATCTTAAATTTACTTTCTTTGCATCATTATAAGAAACTGTGATTTGGTTTTAACTGTT
ATTAACTCTCGTTTTAACACGTTCAATCTAAATCCTTTTGAAATAATCAGATGCGTTGAC
TTATAACATTTTTATCTTTCTTTTTGTCTTGCAGATGCATGTCAATCTGCAGCTTTGGTA
GCTGTGAGTTTGATTAGCTCTGCACGTGTTATCTTCAAGATCGACTCTNAGTATACTGAG
TACTCACCTCAGTATTTGGTGGATAACGTTGGGAAGGAAGAAGTTGAGGGAGAAATGGAT
CAACCAAGCTGTCAGTACCAATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11