Sequences from confirmation amplicon


Confirmation sequences for line 495F04 and hit At1g02610 are shown.
Line ID 495F04
Locus At1g02610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:554108
Primers used for PCR 17nm - 8474, show primer details
   
Sequence Name 56-K117914-0022-495-F04C-17nm
GenBank Accession LN509225 [GenBank]
Sequencing primer gene specific primer, 17nm: 5'-AAAAAGAAGAGCAATTTACCGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117914-0022-495-F04C-17nm
ACCGCATCNATGCATGCTTTATCAACAAAATAACCGAAAACTGCAAAAAGAAAAAATTAT
ATCAATTTAATTAGAGTCACGCCGTAAAAGATGTACCCATATATGGATATGTTATGATAT
GATATTTAGCTCAAATAAAATTGGTTTGGTTGAATTTGGGTATGGCTTGAATATGGGTTA
AAGCTTGGAAAAGACGTTATGGGCTATGTTACCACTTACCACTATGAACAATTTTTTAAT
TTAGTAAACAGACATTCTTTTGGTTCCAAAAGAGGAAACCTTTTACTTTCTTTAGGTTTT
CTTGGTCGACCAAACAACCTCACCGAACCACTTACCACAAATGCATGTATGTGAAAAGAA
TCCTAACAATCATTCTAATCTAATGTCATTATATTTGTCTCTATATATATTTCATAGCAC
CACCAAACTTGAACCATTTCTAACGCAAAACTATTAAAATTAATTTTTTGTCCGCAATGA
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 16-K117913-0022-495-F04C-8409
GenBank Accession LN493615 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117913-0022-495-F04C-8409
CATTTCTCATTGCGGACAAAAAATTAATTTTAATAGTTTTGCGTTAGAACTGGTTCAAGT
TTGGTGGTGCTATGAAATATATATAGAGACAAATATAATGACATTAGATTAGAATGATTG
TTAGGATTCTTTTCACATACATGCATTTGTGGTAAGTGGTTCGGTGAGGTTGTTTGGTCG
ACCAAGAAAACCTAAAGAAAGTAAAAGGTTTCCTCTTTTGGAACCAAAAGAATGTCTGTT
TACTAAATTAAAAAATTGTTCATAGTGGTAAGTGGTAACATAGCCCATAACGTCTTTTCC
AAGCTTTAACCCATATTCAAGCCATACCCAAATTCAACCAAACCAATTTTATTTGAGCTA
AATATCATATCATAACATATCCATATATGGGTACATCTTTTACGGCGTGACTCTAATTAA
ATTGATATAATTTTTTCTTTTTGCAGTTTTCGGTTATTTTGTTGATAAAGCATGCATTTG
ATGCGGTTTATGGAACTGAAGAGTATCCCTATACAATATTCACGGTAAATTGCTCT

Last Updated on 06.12.2018 16:11