Sequences from confirmation amplicon


Confirmation sequences for line 496B03 and hit At1g14410 are shown.
Line ID 496B03
Locus At1g14410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4929939
Primers used for PCR HM68 - 8474, show primer details
   
Sequence Name 75-K101328-0022-496-B03C-8409
GenBank Accession LN513206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101328-0022-496-B03C-8409
GAGTTCCATTTCTGTAACAGACAATGAGAACACCTGCAATTTTGACATGAAGCTTTACCA
AATTCATAATAGAAAAAAGACTGCCTTTTAACACCAAATCCAGTCAAATGGTCTTAACAA
AGTTACAGCTCAAGGATCATCTGAAGTTAAAGAGATAAAACCAAACCTGTTTCTTGCTCC
AATCATATTGTCGTACACCAGCAGAAGGAGCAAACTGGAGCAATAAGAAACCATCTTTTG
ACAACTTGAAAGCTCCAGACTGCAGAAAAACAACAGAAATCAAATGGAAAACTCAAAGCA
TGATTATTCAGTTAGAGCTCTTTGATCTTTAATTTTGGGATAGAGACTTACGTCTAAAGC
CACAAACTCTGGTGCTCTTGGGTCCACTGTTAACGCAGCTTTCCCTTTGTATATCGAATG
ACCCACGTAA
   
Sequence Name 75-K101334-0022-496-B03C-HM68
GenBank Accession LN513207 [GenBank]
Sequencing primer gene specific primer, HM68: 5'-TTTACGTGGGTCATTCGATATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101334-0022-496-B03C-HM68
GAGTTTGTGGCTTTAGACGTAAGTCTCTATCCCAAAATTAAAGATCAAAGAGCTCTAACT
GAATAATCATGCTTTGAGTTTTCCATTTGATTTCTGTTGTTTTTCTGCAGTCTGGAGCTT
TCAAGTTGTCAAAAGATGGTTTCTTATTGCTCCAGTTTGCTCCTTCTGCTGGTGTACGAC
AATATGATTGGAGCAAGAAACAGGTTTGGTTTTATCTCTTTAACTTCAGATGATCCTTGA
GCTGTAACTTTGTTAAGACCATTTGACTGGATTTGGTGTTAAAAGGCAGTCTTTTTTCTA
TTATGAATTTGGTAAAGCTTCATGTCAAAATTGCAGGTGTTCTCATTGTCTGTTACAGAA
ATTGGAACTCTTGTGAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA

Last Updated on 06.12.2018 16:11