Sequences from confirmation amplicon


Confirmation sequences for line 496H02 and hit At1g53680 are shown.
Line ID 496H02
Locus At1g53680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20038494
Primers used for PCR GA69 - 8474, show primer details
   
Sequence Name 46-K101501-0022-496-H02C-8409
GenBank Accession LN505813 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101501-0022-496-H02C-8409
CATTTACATTGTTGTTGAATCAAACCCTGTTCACAGAAAGTCCCTGTTCTTATCCACAAC
AACACACCAATTTCTGAATCGCTCATCCAAGTTCAGTACATCGACGAGACGTGGACTGAT
GCTGCATCTTTCTTGCCCTCTGATCCTCAATCTAGAGCCACTGCAAGGTTCTGGGCTGAT
TACGCTGATAAAACGGTAATTAGTGTTTATAATTCATCTGTTTCTGTTTCGGTTTGCTTT
ATATGTCTACTTGGTTTTGATCAAATTCACCAAAATGCAGATATCATTTGAAGGAGGAAG
AAAGATTTGGGGAAACAAGAAAGGGGAAGAACAAGAGAAAGGCAAGAAAGAGTTTCTTGA
GAGCTTGAAGGTTCTTGAAGCTGAGCTTGGAGACNAATCTNACTTTGG
   
Sequence Name 46-K101505-0022-496-H02C-GA69
GenBank Accession LN505814 [GenBank]
Sequencing primer gene specific primer, GA69: 5'-CCAAAGTAAGATTTGTCTCCAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101505-0022-496-H02C-GA69
CTTAGCTCTCAGAACTCTTTCTTGCCTTTCTCTTGTTCTTCCCCTTTCTTGTTTCCCCAA
ATCTTTCTTCCTCCTTCAAATGATATCTGCATTTTGGTGAATTTGATCAAAACCAAGTAG
ACATATAAAGCAAACCGAAACAGAAACAGATGAATTATAAACACTAATTACCGTTTTATC
AGCGTAATCAGCCCAGAACCTTGCAGTGGCTCTAGATTGAGGATCAGAGGGCAAGAAAGA
TGCAGCATCAGTCCACGTCTCGTCGATGTACTGAACTTGGATGAGCGATTCAGAAATTGG
TGTGTTGTTGTGGATAAGAACAGGGACTTTCTTGTGAACAGGGTTTGATTTCAACAACAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCCGC

Last Updated on 06.12.2018 16:11