Sequences from confirmation amplicon


Confirmation sequences for line 498A09 and hit At2g46810 are shown.
Line ID 498A09
Locus At2g46810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19241015
Primers used for PCR 19ij - 8474, show primer details
   
Sequence Name 51-K113947-0022-498-A09C-19ij
GenBank Accession LN507572 [GenBank]
Sequencing primer gene specific primer, 19ij: 5'-GTAGTGGTTGGTTTCAGAATGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113947-0022-498-A09C-19ij
GGCTTTTCAGTGTCTGGAGAGATAGTAGTGCTAGAAAGCTTGGGTCTTTGAATGACAAAA
ACGGTGAAGGGTCTTCACTTTGTTGCAGCATTTGTAGAAATGGTATTGTTGTGTCTTGCA
GCAAGCTCGGAGTTGAGAGTTGTTGTTCTTCCTCTTCAAGGGCAAAGCTTTGATGATCCT
CTACAGTTTCTTGAACTTGTAGGCTTCTTCTCACATTCACTTTTTGGTCCAAACACTGCA
ACAAAAGAAAAAACAGGGGAAGGAGTTACCTACCAAGTGAGTAAGAAGAGAAAGTGAGAT
TGAGAAGAGAGATATTAAATTTGGGTAGACTAGTGTGGGTTTTGGTTAGTGGGTCAGTCT
AGCGAAACAAAATTAGAATGAAAAATGGCACATATAGATATAAATGGCTTCAATTGTAAA
TGGCTTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
T
   
Sequence Name 23-K113946-0022-498-A09C-8409
GenBank Accession LN496642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113946-0022-498-A09C-8409
ATTTACTGAAGCCATTTACAATTGAAGCCATTTATATCTATATGTGCCATTTTTCATTCT
AATTTTGTTTCGCTAGACTGACCCACTAACCAAAACCCACACTAGTCTACCCAAATTTAA
TATCTCTCTTCTCAATCTCACTTTCTCTTCTTACTCACTTGGTAGGTAACTCCTTCCCCT
GTTTTTTCTTTTGTTGCAGTGTTTGGACCAAAAAGTGAATGTGAGAAGAAGCCTACAAGT
TCAAGAAACTGTAGAGGATCATCAAAGCTTTGCCCTTGAAGAGGAAGAACAACAACTCTC
AACTCCGAGCTTGCTGCAAGACACAACAATACCATTTCTACAAATGCTGCAACAAAGTGA
AGACCCTTCACCGTTTTTGTCATTCAAAGACCCAAGCTTTCTAGCACTACTATCTCTCCA
GACACTTGAAAAGCCTTGGGAACTCGAAAACTACCTCCCACATGAAGTTCCAGAGTTTCA
TTCACCGATCCATTCTGAAACAANCCACTA

Last Updated on 06.12.2018 16:11