Sequences from confirmation amplicon


Confirmation sequences for line 498B03 and hit At2g27170 are shown.
Line ID 498B03
Locus At2g27170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11614415
Primers used for PCR ND15 - 8474, show primer details
   
Sequence Name 17-K032005-022-498-B03C-8409
GenBank Accession LN493742 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K032005-022-498-B03C-8409
GAAGATGCACTTGAGCACTTAATACAGTGGAAAGAGAAATGCAGGATTCTTTGAGGGAAC
TGGAGGCGATTAAGCCTTTGTATGAAAGTCAAGTTGACAAGGAAAACCAGACATCAAAAA
GGTAAGCATATATATAATGTCATTGTAACACGGAAATAGCTTTGTTCATGTTTACAATGA
ATGGGTTGTATGTTGCTATAGGCCTCCTGTTTGAGGGAAGTAATAGTATTAATATCCTAG
AAAGACTACAACGATGATTTTGGGTAAAAGCTTAAATGTTGAATTAAAATGTGAAGTTCT
ACTGTTTGGAGTGATGCATATTGGAATTTGNTTTCTGG
   
Sequence Name 18-K032005-022-498-B03C-ND15
GenBank Accession LN494191 [GenBank]
Sequencing primer gene specific primer, ND15: 5'-ACCAGAAACAAAATTCCAATATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K032005-022-498-B03C-ND15
TTTTATTCACATTTAAGCTTTTACCCAAAATCATCGTTGTAGTCTTTCTAGGATATTAAT
ACTATTACTTCCCTCAAACAGGAGGCCTATAGCAACATACAACCCATTCATTGTAAACAT
GAACAAAGCTATTTCCGTGTTACAATGACATTATATATATGCTTACCTTTTTGATGTCTG
GTTTTCCTTGTCAACTTGACTTTCATACAAAGGCTTAATCGCCTCCAGTTCCCTCAAAGA
ATCCTGCATTTCTCTTTCCACTGTATTAAGTTGCTCAAGTGCATCATTCTGTATTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11