Sequences from confirmation amplicon


Confirmation sequences for line 498E08 and hit At3g63350 are shown.
Line ID 498E08
Locus At3g63350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23399609
Primers used for PCR AQ19 - 8474, show primer details
   
Sequence Name 02-K106588-0022-498-E08C-8409
GenBank Accession LN487046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K106588-0022-498-E08C-8409
ATTGAATATATCCTGCTTTGTGAAGACCAATGTGGTTTGTGTTTGGATCACCAACCATCT
CGAACGTCTTTGTTAGAAAAGGAGAAGGCCCTGCTTCCTGCAATCCCTCCATAGGCACCG
GCGGTGGCATTGACCGTGCTCTGGAGCTTGACGACGGGTCCATCTTGTTAGCAATGCTAA
TGCTAGAAGTCTAATGATCTATGATTGGATTTTTCGTAAATTATAATCCAAGGAAGGAAA
CGATGCATTTGGGGTTCCTGTATTGGAGAAGACCAAAACAGCACAGAACCAAAAGAAGAA
AGAGCGGGAAGTGTATTTTTTCTTTAAAACAGAGTACTCTGCTTTTGCTGGTGGTTAGTT
ACTTAGTTTATGGCAGTTGGGGCAGGAAATGTGGCGAAGACAATGGCCACGTAGGTGACT
TAAATCCATATATATATATAGCGGGGAGCTGGTGATCCTTAAAATTCTTTTAGTCACCAA
GTGGAGTTCGAACTTTCTAGAGCTATTTACCAGAGGGAGGAAGGGTGCATATCTTCTTAG
AAAGTTCCGGAACTTATACTTTTGAGGAGATTAAGGTAAAATCGTATGTGGTGGATCCCA
TGATTTACATAT
   
Sequence Name 18-K106589-0022-498-E08C-AQ19
GenBank Accession LN494319 [GenBank]
Sequencing primer gene specific primer, AQ19: 5'-GATATGTAAATCATGGGATCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106589-0022-498-E08C-AQ19
GTTCCGGAACTTTCTAGAAGATATGCACCCTTCCTCCCTCTGGTAAATAGCTCTAGAAAG
TTCGAACTCCACTTGGTGACTAAAAGAATTTTAAGGATCACCAGCTCCCCGCTATATATA
TATATGGATTTAAGTCACCTACGTGGCCATTGTCTTCGCCACATTTCCTGCCCCAACTGC
CATAAACTAAGTAACTAACCACCAGCAAAAGCAGAGTACTCTGTTTTAAAGAAAAAATAC
ACTTCCCGCTCTTTCTTCTTTTGGTTCTGTGCTGTTTTGGTCTTCTCCAATACAGGAACC
CCAAATGCATCGTTTCCTTCCTTGGATTATAATTTACGAAAAATCCAATCATAGATCATT
AGACTTCTAGCATTAGCATTGCTAACAAGATGGACCCGTCGTCAAGCTCCAGAGCACGGT
CAATGCCACCGCCGGTGCCTATGGAGGGATTGCAGGAAGCAGGGCCTTCTCCTTTTCTAA
CAAAGACGTTCGAGATGGTTGGTGATCCAAACACAAACCACATTGTGTCTTCACAAAGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTA
Primers used for PCR CC67 - 8474, show primer details
   
Sequence Name 03-K106588-0022-498-E08C-OriL
GenBank Accession LN487499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K106588-0022-498-E08C-OriL
TCATTGAATATATCCTGCTTTGTGAAGACACAATGTGGTTTGTGTTTGGATCACCAACCA
TCTCGAACGTCTTTGTTAGAAAAGGAGAAGGCCCTGCTTCCTGCAATCCCTCCATAGGCA
CCGGCGGTGGCATTGACCGTGCTCTGGAGCTTGACGACGGGTCCATCTTGTTAGCAATGC
TAATGCTAGAAGTCTAATGATCTATGATTGGATTTTTCGTAAATTATAATCCAAGGAAGG
AAACGATGCATTTGGGGTTCCTGTATTGGAGAAGACCAAAACAGCACAGAACCAAAAGAA
GAAAGAGCGGGAAGTGTATTTTTTCTTTAAAACAGAGTACTCTGCTTTTGCTGGTGGTTA
GTTACTTAGTTTATGGCAGTTGGGGCAGGAAATGTGGCGAAGACAATGGCCACGTAGGTG
ACTTAAATCCATATATATATATAGCGGGGAGCTGGTGATCCTTAAAATTCTTTTAGTCAC
CAAGTGGAGTTCGAACTTTCTAGAGCTATTTACCAGAGGGAGGAAGGGTGCATATCTTCT
TAGAAAGTTCCGGAACTTATACTTTTGAGGAGATTAAGGTAAAATCGTATGTGGTGGATC
CCATGATTTACATATCACCTTCCCCCCCCCCCCTT
   
Sequence Name 19-K106589-0022-498-E08C-CC67
GenBank Accession LN494773 [GenBank]
Sequencing primer gene specific primer, CC67: 5'-GATATGTAAATCATGGGATCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K106589-0022-498-E08C-CC67
GTTCCGGAACTTTCTAGAAGATATGCACCCTTCCTCCCTCTCCCTTTAAAATCTAGAAAG
TTCGAACTCCACTTGGTGACTACCCCTATATAAGGATCACCAGCTCCCCGCTACATATAT
ATATGGATTTAAGTCACCTACTTGGCCATTGTCTTCGCCACCTTTCCTGCCCCACCTGCC
ACAAACTATGTACCTAACCACGAGCAAAAGCGGAGTACTCTGTTATAAAGAAAAAATACA
CTTCCCGCTCTTTCTTCTTGCGGTTCTGTGCTGATTTGGTCTTCCCCACTACAGGAACCC
CAAATGCAGCGCTTCCTTCCTTGGAT

Last Updated on 06.12.2018 16:11