Sequences from confirmation amplicon


Confirmation sequences for line 498G08 and hit At3g15460 are shown.
Line ID 498G08
Locus At3g15460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5215365
Primers used for PCR CM41 - 8474, show primer details
   
Sequence Name 11-K104123-0022-498-G08C-CM41
GenBank Accession LN491088 [GenBank]
Sequencing primer gene specific primer, CM41: 5'-TGTTGAAATTTACATTGGGTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104123-0022-498-G08C-CM41
AATCATGGAGTAACTTTGTTAATTCGTTGGAAGGTATCGACATTTGATGTTGAACATGGT
GTCACTTTTACCTCACTGTAAGAAGGATAGTAAGGTAGAAGCTAAGAGCAGTAGAGGTGC
CACTCTTAATGAGCTCATCGAGCTTAAGGGCTCTTCTTCCTGTTTGTTTTTCGAGGTAAA
GTTTCAATCTTTTTTTTGGCTACCTTAATTCCAGAACCAGTTACTGTTCTATGGTCTGCT
CTTCGTAAGGGGTTTGGTAATCATATTCGTATCAATCTTCTTAATTTCTTTTTCCATGTA
TTTACCTATTTGGTATGAAATGTAACTTGGTAAAACTTTCGTACCATTGATGATAGAAAC
AAAGAGCATTACAGATTGAATTCTCTTTTGTCTGGTTCAAATTGGGCTCTCTAGTCTCTC
TGTTTTAAATGTCAGCATGTCTGGATTTACTTGATTTCAGCATGTCTGTAGAGTATTAAA
CTGCAAAGTCAATGTATTCTTGATTTCTTCCCAGAAACTGAAGTTAGTACGTGTTTGTTT
GTGTTCGTCTTCAGTGTAGGAAGCACAAAGATCTTTATATAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTAAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 27-K104124-0022-498-G08C-8409
GenBank Accession LN498360 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104124-0022-498-G08C-8409
GCCATTTATATAAAGATCTTNTGTGCTTCCTACACTGAAGACGAACACAAACAAACACGT
ACTAACTTCAGTTTCTGGGAAGAAATCAAGAATACATTGACTTTGCAGTTTAATACTCTA
CAGACATGCTGAAATCAAGTAAATCCAGACATGCTGACATTTAAAACAGAGAGACTAGAG
AGCCCAATTTGAACCAGACAAAAGAGAATTCAATCTGTAATGCTCTTTGTTTCTATCATC
AATGGTACGAAAGTTTTACCAAGTTACATTTCATACCAAATAGGTAAATACATGGAAAAA
GAAATTAAGAAGATTGATACGAATATGATTACCAAACCCCTTACGAAGAGCAGACCATAG
AACAGTAACTGGTTCTGGAATTAAGGTAGCCAAAAAAAAGATTGAAACTTTACCTCGAAA
AACAAACAGGAAGAAGAGCCCTTAAGCTCGATGAGCTCATTAAGAGTGGCACCTCTACTG
CTCTTAGCTTCTACCTTACTATCCTTCTTACAGTGAGGTAAAAGTGACACCATGTTCAAC
ATCAAATGTCGATACCTTCCAACGAATTAACAAAGTTACTCCATGATTCATAACCATTAA
ACCGAATCAAACCCAATGAAATTTTTCAAC

Last Updated on 06.12.2018 16:11