Sequences from confirmation amplicon


Confirmation sequences for line 499C06 and hit At2g44910 are shown.
Line ID 499C06
Locus At2g44910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18518568
Primers used for PCR 26wc - 8474, show primer details
   
Sequence Name 49-K118511-0022-499-C06C-26wc
GenBank Accession LN506990 [GenBank]
Sequencing primer gene specific primer, 26wc: 5'-GGTCAGATTATCGCAACATCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118511-0022-499-C06C-26wc
CTTCGTCCTATGTACGTACAAATAATAAATCAAAATCACCTTAATTAGTGAAGTGAACAG
AAATAAAAGTTTAAAACTGATAAGAAACAAAAAAGGACAAAAGTAGATTTTATTTGTACG
CATACGCATGCATGTGTCTTTAAGGCTGTAGTAAAAAGTAAGAAACTCCATGTGACAGTA
CAGAGGCAGAGATCTTATTAGGATTTTCTTTTAGGACAGGGAACTGAAATCGCTTTTGGG
TCATAAAAGGACAATTATATCCCCATCATAGCAATTACTAAGGTTCTGTGAAACCAAATT
TCCTATAATACCCCTAGAAAATAAAGATAATTTCTTTACCTTGCCCTACGGTTCTGAAAC
CACACTTCAACTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCACAATTTATTATAANNAGGAAATTTGGTTTCNCAGAACCTTNGTTAATT
GCTATGATGGGGATATAATTTGTCCTTTTATGACCCAAAA
   
Sequence Name 21-K118510-0022-499-C06C-8409
GenBank Accession LN495868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118510-0022-499-C06C-8409
CATTTACAGTTGAAGTGTGGTTTCAGAACCGTAGGGCAAGGTAAAGAAATTATCTTTATT
TTCTAGGGGTATTATAGGAAATTTGGTTTCACAGAACCTTAGTAATTGCTATGATGGGGA
TATAATTGTCCTTTTATGACCCAAAAGCGATTTCAGTTCCCTGTCCTAAAAGAAAATCCT
AATAAGATCTCTGCCTCTGTACTGTCACATGGAGTTTCTTACTTTTTACTACAGCCTTAA
AGACACATGCATGCGTATGCGTACAAATAAAATCTACTTTTGTCCTTTTTTGTTTCTTAT
CAGTTTTAAACTTTTATTTCTGTTCACTTCACTAATTAAGGTGATTTTGATTTATTATTT
GTACGTACATTAGGACGAAGCTGAAACAAACGGAGGTTGATTGTGAGTATTTAAAGAGAT
GTTGCGATAATCTGACCAAC

Last Updated on 06.12.2018 16:11