Sequences from confirmation amplicon


Confirmation sequences for line 499H10 and hit At1g24793 are shown.
Line ID 499H10
Locus At1g24793 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8767783
Primers used for PCR 43oc - 8474, show primer details
   
Sequence Name 42-K114678-0022-499-H10C-43oc
GenBank Accession LN504560 [GenBank]
Sequencing primer gene specific primer, 43oc: 5'-CTGTCACTTGGTTAGTGTTGCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114678-0022-499-H10C-43oc
AGTCTATAAACTAAGAGCTACACTGCACAGCAAGATGTTGACAATCTCTATAGCATACAA
ATCCAGTCAAAATAGACAATACGAGACATTGATTCGACCTATGATTACTCAAAATGTATG
TATTCAGGTAACAAACCTCGACTTCTCGATCATCGGAACTTTCGCTCTCGATTTGAATCC
TGCAATTATCAACTCCCTTCGCCTCAAGAGCTGATAACAAATGCTCAACGGTTCGGATTT
TGAGCTCATCTTTCAACAGCGTCGTGCAAAGAGGCGATTCCTGAGCAAATTCAATCGACG
CAGGGATGAATCTGGATCGAAACTCGAAGAACCTACCAGCTCCGGCGATCTCCGGATTTA
GCTTCACCGTCGAGAACTTACCGGAGTGCAGAGATTTTCCTTTCACTTCCACAGATCCTG
CGAGAGTTTGCTGTAATCGTCCACTCAAAATGACGATNAAAAAACAAAAAAAAGAAACAC
ACTCAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
C
   
Sequence Name 18-K114677-0022-499-H10C-8409
GenBank Accession LN494413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114677-0022-499-H10C-8409
ATTTTGAGTGTGTTTCTTTTTTTTGTTTTTTATCGTCATTTTGAGTGGACGATTACAGCA
AACTCTCGCAGGATCTGTGGAAGTGAAAGGAAAATCTCTGCACTCCGGTAAGTTCTCGAC
GGTGAAGCTAAATCCGGAGATCGCCGGAGCTGGTAGGTTCTTCGAGTTTCGATCCAGATT
CATCCCTGCGTCGATTGAATTTGCTCAGGAATCGCCTCTTTGCACGACGCTGTTGAAAGA
TGAGCTCAAAATCCGAACCGTTGAGCATTTGTTATCAGCTCTTGAGGCGAAGGGAGTTGA
TAATTGCAGGATTCAAATCGAGAGCGAAAGTTCCGATGATCGAGAAGTCGAGGTTTGTTA
CCTGAATACATACATTTTGAGTAATCATAGGTCGAATCAATGTCTCGTATTGTCTATTTT
GACTGGATTTGTATGCTATAGAGATTGTCAACATCTTGCTGTGCAGTGTAGCTCTTAGTT
TTATAGACTTGGTACACTGGAGGAGATGATGAGTGTAGGCAACACTAACAA

Last Updated on 06.12.2018 16:11