Sequences from confirmation amplicon


Confirmation sequences for line 499H12 and hit At2g46160 are shown.
Line ID 499H12
Locus At2g46160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18963415
Primers used for PCR CC70 - 8474, show primer details
   
Sequence Name 67-K106292-0022-499-H12C-OriL
GenBank Accession LN511821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K106292-0022-499-H12C-OriL
GGAATGAGCCATTTACAATTTACCGCATAATCCTCCTCCGCCTTCGGTTTGTCGTTGATT
GCTGCCCATTGTATTTACATTTAAAATTGGTAATTGATCAAGCCGTCATCAACTCATACC
CTAAGTTCCACTTTTCCAAAGATACCTCCGCCGCGTCCTCAGATGGCTTTGGTGGCGGAG
GAGACACAACGTGCTCGATCTGTTTATGTGAGTATAAAGAAGCGGAGATGCTGAGGATGA
TGCCGGAGTGTAAACACTACTTCCATTTGTGTTGCCTTGATGCGTGGCTTAAACTCAACG
GATCTTGTCCCGTTTGTCGGAACTCGCCGCTTCCAACGCCGACTTCAACGCCTCTGTCAA
CGCCTTTGTCAGAAGTTGTGCCTCTCTCCCAGTACGCGGCCGATCGGAGGAGAGCAAGAA
GATGATATTATTATTACATAGATCCATTCGCCTAAATTAGGGTAATTCCTATTTATTATT
TTTGCTCCGTTTCATTACAACTTTACTGATTTGTAATTAAAAAAAATCCGTCAAAAGTGG
AGTTTGCCATTAATTTCATCGAAATGAAATCTAGTTTGAATCGTTTACTAACCTTACAAA
ATTTATCCTTTTCTATTGGCGTTTTTGAACGTTTTTTTCCGTTTCCCCTATCAATATGAG
CGGTTC
   
Sequence Name 67-K106293-0022-499-H12C-CC70
GenBank Accession LN511822 [GenBank]
Sequencing primer gene specific primer, CC70: 5'-CGTATTTTATTTTGAACCGCTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K106293-0022-499-H12C-CC70
CGCCATAGAAAAGGATAAATTTTGTAAGGTTAGTAAACGATTCAAACTAGATTTCATTTC
GATGAAATTAATGGCAAACTCCACTTTTGACGGATTTTTTTTAATTACAAATCAGTAAAG
TTGTAATGAAACGGAGCAAAAATAATAAATAGGAATTACCCTAATTTAGGCGAATGGATC
TATGTAATAATAATATCATCTTCTTGCTCTCCTCCGATCGGCCGCGTACTGGGAGAGAGG
CACAACTTCTGACAAAGGCGTTGACAGAGGCGTTGAAGTCGGCGTTGGAAGCGGCGAGTT
CCGACAAACGGGACAAGATCCGTTGAGTTTAAGCCACGCATCAAGGCAACACAAATGGAA
GTAGTGTTTACACTCCGGCATCATCCTCAGCATCTCCGCTTCTTTATACTCACATAAACA
GATCGAGCACGTTGTGTCTCCTCCGCCACCAAAGCCATCTGAGGACGCGGCGGAGGTATC
TTTGGAAAAGTGGAACTTAGGGTATGAGTTGATGACGGCTTGATCAATTACCAATTTTAA
ATGTAAATACAATGGGCAGCAATCAACGACAAACCGAAGGCGGAGGAGGATTATGCGGTT
AAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
Primers used for PCR CC71 - 8474, show primer details
   
Sequence Name 66-K106292-0022-499-H12C-8409
GenBank Accession LN511617 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106292-0022-499-H12C-8409
GGACTGAGCCTTCTTTTTGGAATGTCCACGACATCACCTGCTTCGAGATCTTCATTGTCT
TCCTCGGCGACAAAGATTATGCGGGGGAGGATTACACTTCCGCCACGATCGCCTGTGGAT
TCCACGGCGGTTGTGCGGCGGCGAGAGTCACGGCAACAGATGTATGAGGATAAAAGGACG
GTCGAGAGAAGGACGAGAAAACCGAGAGCTATGGCTATGGAGTAGCCAAAGCCTAGAGTT
GTGAGGTAAGAGTGGTTGCCTGCTTGTGGTGGCGACGGTGGAAGGGGAGGGTGGACTAGG
AAGACGGAGGCGGAGGAGGCGAGTGTTGACATTTGATGGTAAAGAAGAAGAAGACTGGGT
CCATGTGGGAGAGAGAGAGAAGAGAGTGATAGAGATTTTTATTTTTTTATTTTTTTGAAA
ATTTAATTTTAATTAATTTAGTTAGGGGAAGAGAAAAAGAGGTGGGTGGGAATTGCAGCT
GATGCATTTAATATTGTTTGGAAACTGAAATTTGCATTTATTAGTAAGTTAGGTTTTTAT
TAATTAAGTGGCCCAACCCCAAGGAATAATATATTTTCTTGTTTTACTTTGTAATTTTTT
TTGTTTATTGTATAAAATGTGTTCGAGCCATGTAACGTTGACTGTTT
   
Sequence Name 66-K106293-0022-499-H12C-CC71
GenBank Accession LN511618 [GenBank]
Sequencing primer gene specific primer, CC71: 5'-TAAACAGTCAACGTTACATGGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106293-0022-499-H12C-CC71
ACAAAAATTACAAGTAAAACAAGAAAATATATTATTCCTTGGGGTTGGGCCACTTAATTA
ATAAAAACCTAACTTACTAATAAATGCAAATTTCAGTTTCCAAACAATATTAAATGCATC
AGCTGCAATTCCCACCCACCTCTTTTTCTCTTCCCCTAACTAAATTAATTAAAATTAAAT
TTTCAAAAAAATAAAAAAATAAAAATCTCTATCACTCTCTTCTCTCTCTCTCCCACATGG
ACCCAGTCTTCTTCTTCTTTACCATCAAATGTCAACACTCGCCTCCTCCGCCTCCGTCTT
CCTAGTCCACCCTCCCCTTCCACCGTCGCCACCACAAGCAGGCAACCACTCTTACCTCAC
AACTCTAGGCTTTGGCTACTCCATAGCCATAGCTCTCGGTTTTCTCGTCCTTCTCTCGAC
CGTCCTTTTATCCTCATACATCTGTTGCCGTGACTCTCGCCGCCGCACAACCGCCGTGGA
ATCCACAGGCGATCGTGGCGGAAGTGTAATCCTCCCCCGCATAATCTTTGTCGCCGAGGA
AGACAATGAAGATCTCGAAGCAGGTGATGTCGTGGACATTTCCAAAAAGAAAGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11