Sequences from confirmation amplicon


Confirmation sequences for line 502H09 and hit At5g24650 are shown.
Line ID 502H09
Locus At5g24650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8437720
Primers used for PCR 13ie - 8474, show primer details
   
Sequence Name 47-K112758-0022-502-H09C-13ie
GenBank Accession LN506243 [GenBank]
Sequencing primer gene specific primer, 13ie: 5'-GAAAAACACTCCTTGAAAAACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K112758-0022-502-H09C-13ie
ATCCTGCGCTCACAAGAGAATATGCAACTCCAGATCCGAATGCTGCGACCACACTACACA
GTCGAAAAACATGACTCTTTTTCACACATCGAGTTTCAACAAACAAACAAGCTACAAATA
AGCCACTGAGAATCTGCACGCATCAAAGATTGTATCTAGATTCTTTCAAAAGTAAACACA
CACAAGTGGATAAAGCCCTACATGAACAGTGAATCATTATAAGTATCAGAATGATCATAA
CAGCTGATTCGAGAAAGAGATTGTAACTCTATGAAGCAATAAATGATGAAAAAACAAACG
AAGTCATCTTTTTTTCCTGTCATGGAATCAATCTTGTGACAGAGAATCTGATTAAGACCT
GTAACTTATGTCAGCTATTCAAAATCTTACTGTTGATCAGAAAAATGACAATTGCAAAAA
CATAAACACTAGTTGCAGTTTTGCAGACACTAATTGTCTCTAAAAACAGCTCCAGAATTC
AGTTTCTAGAATTCGTTAAGTCCTAGATCATTGATGAAATGTATTGAAATGTAAGTTTTC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCC
   
Sequence Name 07-K112757-0022-502-H09C-8409
GenBank Accession LN489347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112757-0022-502-H09C-8409
CATTTCATTGAATATATCCTGAAACTTACATTTCATACATTTCATCAATGATCTAGGACT
TAACGAATTCTAGAAACTGAATTCTGGAGCTGTTTTTAGAGACAATTAGTGTCTGCAAAA
CTGCAACTAGTGTTTATGTTTTTGCAATTGTCATTTTTCTGATCAACAGTAAGATTTTGA
ATAGCTGACATAAGTTACAGGTCTTAATCAGATTCTCTGTCACAAGATTGATTCCATGAC
AGGAAAAAAAGATGACTTCGTTTGTTTTTTCATCATTTATTGCTTCATAGAGTTACAATC
TCTTTCTCGAATCAGCTGTTATGATCATTCTGATACTTATAATGATTCACTGTTCATGTA
GGGCTTTATCCACTTGTGTGTGTTTACTTTTGAAAGAATCTAGATACAATCTTTGATGCG
TGCAGATTCTCAGTGGCTTATTTGTAGCTTGTTTGTTTGTTGAAACTCGATGTGTGAAAA
AGAGTCATGTTTTTCGACTGTGTAGTGTGGTCGCAGCATTCGGATCTGGAGTTGCATATT
CTCTTGTGAGCGCAGGATTACAAGGACAGCCTATGAATGCAATCACCACTGCTGCTGGTT
TCGCTGTTTTTCAAGGAGTGGTTTTT

Last Updated on 06.12.2018 16:11