Sequences from confirmation amplicon


Confirmation sequences for line 503A04 and hit At4g19110 are shown.
Line ID 503A04
Locus At4g19110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10457388
Primers used for PCR GA60 - 8474, show primer details
   
Sequence Name 50-K101502-0022-503-A04C-8409
GenBank Accession LN507110 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101502-0022-503-A04C-8409
GACTAACTCATAAGCCACAAATCAAGGGGCCCGATGAAAAAGATATTAAAAAACAAGTTG
AGAGAAAACTAAGCTACCTGTCCATCTTTTTTTTTCTGGTTAAGCAAAGTAGCCTCTTTA
TATTATGTAAGTAAAGAACTTCTGAAAGATTTATATAGCATTAATCTTCCAGTCGTTCGT
AGGAAGTACTTGACACCAAGGCAGTAAAATCAATGATTCTCCAGCTGGGTAGACTGACCG
TTATTTACTTCGAACATAAACGGGTTGACCATTTAGGCAAAAGAAAGAGAGCAAGAGAAG
AGGAAAACACATACTGAGAACTCAAAGACGCGAAGGAACCAACAAGAAAGACTGTTTTGG
CTAATAGGAAGGACCCTATGCTCACAACATCTCACTCAGGGGTTCAGACTCAAGGAAACG
ACATTTGTAAACTGAAAAAGAAAAGAAATTGATGTATCTNTCTTCAACCAGCACCCATAA
G
   
Sequence Name 50-K101506-0022-503-A04C-GA60
GenBank Accession LN507111 [GenBank]
Sequencing primer gene specific primer, GA60: 5'-ATATCTTGTTCTTATGGGTGCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101506-0022-503-A04C-GA60
TTTCTTTTTTTTTCGTTTACAAATGTCGTTTCCTTGAGTCTGAACCCCTGAGTGAGATGT
TGTGAGCATAGGGTCCTTCCTATTAGCCAAAACAGTCTTTCTTGTTGGTTCCTTCGCGTC
TTTGAGTTCTCAGTATGTGTTTTCCTCTTCTCTTGCTCTCTTTCTTTTGCCTAAATGGTC
AACCCGTTTATGTTCGAAGTAAATAACGGTCAGTCTACCCAGCTGGAGAATCATTGATTT
TACTGCCTTGGTGTCAAGTACTTCCTACGAACGACTGGAAGATTAATGCTATATAAATCT
TTCAGAAGTTCTTTACTTACATAATATAAAGAGGCTACTTTGCTTAACCAGAAAAAAAAA
GATGGACAGGTAGCTTAGTTTTCTCTCAACTTGTTTTTTAATATCTTTTTCATCGGGCCC
CTTGATTTGTGGCTTATGAGTTTAGTTCTCAATGCCTTATGAATTTCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAG

Last Updated on 06.12.2018 16:11