Sequences from confirmation amplicon


Confirmation sequences for line 503A10 and hit At1g14010 are shown.
Line ID 503A10
Locus At1g14010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4801337
Primers used for PCR 26ny - 8474, show primer details
   
Sequence Name 03-K114626-0022-503-A10C-26ny
GenBank Accession LN487589 [GenBank]
Sequencing primer gene specific primer, 26ny: 5'-AAACAAGATCCAACCATAACAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114626-0022-503-A10C-26ny
CTCTGCATCTCTTTAAGAGGCTATGTTACATTTCAAGCTAGTCTTGCTCTGTAGTATGTT
AAAGCCTAGTCCTATCTACCACATTTCAAGATAGTAATTTTCTGCGTCTGTTAAAGCCTA
GTCCTATCCACCTTCATTTAGCAGGGAAAGAACTCCTGAATGAGCTGAAGAAATCGATGG
GGTTCAAAGGTGAAACTAATGAAGCTATTTTCTCATATGCAAGACTGCAGACACAGGTCA
GTAACACACGAGCCCTGTCAGCTTAATTCATGGCCTTGTTCTAACCAAACCAATGCACCA
ACATAACCTATAAACCAAGACTACATAGCATTTCTCACTTCAGATATAACTTTCCACTTT
CCAGCACTATGTCTTGGTGTAACTTAGGCAGCTATCAAATTCTCACACATCCGGCGCAAT
GCTCTATTGTTCATGTCATTAGAAGCATCAAAATCTATTTCCTCTAAAGAGATTAGTTTG
ACTAAGTTAAGACTTCAAGAGTAAAGAAAAAGCGAAAAACTTACTTCGACTTGGCTCTTC
TTTGCCACATTCGACCAATCCTTGGTATGAATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCANAAATGTAGATGTCCC
   
Sequence Name 03-K114625-0022-503-A10C-8409
GenBank Accession LN487588 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114625-0022-503-A10C-8409
TTGAATATTCATACCAAGGATTGGTCGAATGTGGCAAAGAAGAGCCAAGTCGAAGTAAGT
TTTTCGCTTTTTCTTTACTCTTGAAGTCTTAACTTAGTCAAACTAATCTCTTTAGAGGAA
ATAGATTTTGATGCTTCTAATGACATGAACAATAGAGCATTGCGCCGGATGTGTGAGAAT
TTGATAGCTGCCTAAGTTACACCAAGACATAGTGCTGGAAAGTGGAAAGTTATATCTGAA
GTGAGAAATGCTATGTAGTCTTGGTTTATAGGTTATGTTGGTGCATTGGTTTGGTTAGAA
CAAGGCCATGAATTAAGCTGACAGGGCTCGTGTGTTACTGACCTGTGTCTGCAGTCTTGC
ATATGAGAAAATAGCTTCATTAGTTTCACCTTTGAACCCCATCGATTTCTTCAGCTCATT
CAGGAGTTCTTTCCCTGCTAAATGAAGGTGGATAGGACTAGGCTTTAACAGACGCAGAAN
ATTACTATCTTGAAATGTGGTAGATAGGACTAGGCTTTAACATACTACAGAGCAAGACTA
GCTTGAAATGTAACATAGCCTCTTAAAGAGATTGCAGAGTTTTTGAAGGAATGTTTTGTT
AACCTTGTTATG

Last Updated on 06.12.2018 16:11