Sequences from confirmation amplicon


Confirmation sequences for line 503G03 and hit At2g39230 are shown.
Line ID 503G03
Locus At2g39230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16381643
Primers used for PCR 69qa - 8474, show primer details
   
Sequence Name 45-K115481-0022-503-G03C-69qa
GenBank Accession LN505637 [GenBank]
Sequencing primer gene specific primer, 69qa: 5'-CAAAGCAATCTACAGCATAATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115481-0022-503-G03C-69qa
GGGTAAGTCAAAACCAAACCGTTTAGACGAATCAACCAAATTGTTAACCATTACGTTTGG
AATTAGCGTTGGATTGTTCGAAACGAACATTACGAGCAAATTGCTGGCACGATCGTGAGT
GTGAATAGAGCTTAGAAGGATATGAATAAGTACCCAGAAGACATCTCCACCTTCACAGAG
ACGATGTAAAGGCTTGACCCAATTGCAGTACTGAAGAGCTGAGACTGGGTCGTTTCTACG
ACCTAGAAGCACTTCGATTACACGAGCGTCGTCCACATGATTCTCAGTCTCAGAGGTTGA
AGTTAAAGGTGAATGATTTGGTACAGAGGTTTTATGAGGAAATTCTAGTGTTGAGATGGT
TTCAGTAGTGGAATCGGAAATGTCTCGACTTTGATTATCCGGTGGATGGAGAAATCGAGT
CTGTGAAGAAATGGGTTTTGGATTTATAAAACGATGGAGAAAAATAGGTGGACGAAATCG
TTTGGAAACCATGAAAGTAGTCATTTTTTGTTCTCAGCTAGCTTCAGCTTGTAGAACACG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 17-K115480-0022-503-G03C-8409
GenBank Accession LN493986 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115480-0022-503-G03C-8409
AAAAAGACTACTTTTCATGGTTTTCAAACGATTTCCGTCCACCTATTTTTCTCCATCGTT
TTATAAATCCAAAACCCATTTCTTCACAGACTCGATTTCTCCATCCACCGGATAATCAAA
GTCGAGACATTTCCGATTCCACTACTGAAACCATCTCAACACTAGAATTTCCTCATAAAA
CCTCTGTACCAAATCATTCACCTTTAACTTCAACCTCTGAGACTGAGAATCATGTGGACG
ACGCTCGTGTAATCGAAGTGCTTCTAGGTCGTAGAAACGACCCAGTCTCAGCTCTTCAGT
ACTGCAATTGGGTCAAGCCTTTACATCGTCTCTGTGAAGGTGGAGATGTCTTCTGGGTAC
TTATTCATATCCTTCTAAGCTCTATTCACACTCACGATCGTGCCAGCAATTTGCTCGTAA
TGTTCGTTTCGAACAATCCAACGCTAATTCCAAACGTAATGGTTAACAATTTGGTTGATT
CGTCTAAACGGTTTGGTTTTGAACTTACCCCACGAGCTTTCAATTACTTGTTGAATGCTT
ATATTCGAAACAAGCGAATGGATTATGC

Last Updated on 06.12.2018 16:11