Sequences from confirmation amplicon


Confirmation sequences for line 504C09 and hit At3g15220 are shown.
Line ID 504C09
Locus At3g15220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5128444
Primers used for PCR 16wm - 8474, show primer details
   
Sequence Name 82-K118585-0022-504-C09C-16wm
GenBank Accession LN514241 [GenBank]
Sequencing primer gene specific primer, 16wm: 5'-AGAATCCGATGATAAATCCAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118585-0022-504-C09C-16wm
AGCATATAAGATTATTGAAGAATGAAGGCAATTCAACGAGATAACCCTTAAAGATAATAA
AACTGATTAAAGGGGATATATAAACCTCCGCGAGATTTGCGTTGCTATCTTCTGATGCAC
TCGAAGTTCTCTCTTGAATCCCTAGTCTAGATTTCGGGGTTCGAGGTGATCCAGAGTCAT
CGTACTGTCCACGGACAACAACAGTTCCACTTGCTGAAGCATCATCAAAAGCTGCATACC
TGCCGCTAGACTACAAAGTAGAAATGGAGTCATTTATAATACACCAGGCCTAATTAATTT
CTGTATAAGATGTTAAGAAGGTGCCAGAAATTTCATACCCCAGAGCTTGGTTCACGGAAT
ACTGAAGATGATTGAGAACTTCTTGGGGTTCTTATGACAACAGTTCCCGATCCACTGAGC
GATGAATCGTCCTGGATTCGGAAGGACAAGGTAAACATATTTTTATCTTGTTCTCATTCT
TAAGAATACACAGATATAAGGAGAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 52-K118584-0022-504-C09C-8409
GenBank Accession LN507963 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118584-0022-504-C09C-8409
ATTTACATTGAATAATTTCTCCTTATATCTGTGTATTCTTAAGAATGAGAACAAGATAAA
AATATGTTTACCTTGTCCTTCCGAATCCAGGACGATTCATCGCTCAGTGGATCGGGAACT
GTTGTCATAAGAACCCCAAGAAGTTCTCAATCATCTTCAGTATTCCGTGAACCAAGCTCT
GGGGTATGAAATTTCTGGCACCTTCTTAACATCTTATACAGAAATTAATTAGGCCTGGTG
TATTATAAATGACTCCATTTCTACTTTGTAGTCTAGCGGCAGGTATGCAGCTTTTGATGA
TGCTTCAGCAAGTGGAACTGTTGTTGTCCGTGGACAGTACGATGACTCTGGATCACCTCG
AACCCCGAAATCTAGACTAGGGATTCAAGAGAGAACTTCGAGTGCATCAGAAGATAGCAA
CGCAAATCTCGCGGAGGTTTATATATCCCCTTTAATCATTTTATTATCTTTAAGGGTTAT
CTCGTTGAATTGCCTTCATTCTTCAATATATCTTTATATTGCTTTAGATATCTTCTGTTC
ATCTGGATTTATCACCCGGATTC

Last Updated on 06.12.2018 16:11