Sequences from confirmation amplicon


Confirmation sequences for line 504F12 and hit At4g27610 are shown.
Line ID 504F12
Locus At4g27610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13786209
Primers used for PCR JC11 - 8474, show primer details
   
Sequence Name 05-K101743-0022-504-F12C-8409
GenBank Accession LN488296 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101743-0022-504-F12C-8409
AGATTTCTCCATATTGACATCATACTCATTTACTCTTTCTTGGGGGAAGTTCCAGGCGAT
CAGGAGAAGTTGCCCACATGACTCCATTCTCTTTGCTCGATGGAGCAGGAACTGTCACAA
GAGGCAGTGAAGTGTCAGATGCGGATATCATTTGCTCGCTTGTCACCTCTTTTCCTTCCA
CACTCTCAGTTCCTATAAAAATAAAGAAGCAAAGGCTGATAAGATCCATACACGACTGTT
TGCTCTGGACTACATGAATATTAGGATTCAGAGATTTTCCAAAGAGAAAACATAACTCAG
GTCTGGATCTAATAGTACAAAACTTGTAACTATGCTTAACGAGGTCTTGGTAGATAACAA
AACTAGTAACTGTTTTTAGCATTCAGCCACTAGACACAGCCATCAATGGAGAAAACCTGC
TTCCCTAGTTGCCAGCCCAATTGATGCAAATTAATGGTAATATGCCACAACGAAACAAGA
GATATTCGATTCAACCAGTTATTGCAAGGTGGCAAATCTTGTAACTATTTTACTCATTCC
ATAACTTCGAATTGGATTCCCCTAGTTGACAAATACATCATGAGGTAAGATAACAGAGGC
TAGAACTCTATAACAACATCTACACATTCTCCCAACTAGATNTTACTATATATCACACAT
CGTTNTAACTTCATGAAAGGAAACCCAGTAAGACATGATGCGAGACCAAGAAACAAGCAT
ATAGCGTTTGTTTAGTAGATTGAACAAATATGTATGAGGCANAACAGAANGTTACAAAGT
CACACCTGGAATGGAGC
   
Sequence Name 05-K101749-0022-504-F12C-JC11
GenBank Accession LN488297 [GenBank]
Sequencing primer gene specific primer, JC11: 5'-AAATAATGATTTTTGTTGCTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101749-0022-504-F12C-JC11
ACTTTCGTTTTGCCTCAACATATTTGTTCATCTACTAAACAAACGCTATATGCTTGTTTC
TTGGTCTCGCATCATGTCTTACTGGGTTTCCTTTCATGAAGTTAAAACGATGTGTGATAT
ATAGTAAAATCTAGTTGGGAGAATGTGTAGATGTTGTTATAGAGTTCTAGCCTCTGTTAT
CTTACCTCATGATGTATTTGTCAACTAGGGGAATCCAATTCGAAGTTATGGAATGAGTAA
AATAGTTACAAGATTTGCCACCTTGCAATAACTGGTTGAATCGAATATCTCTTGTTTCGT
TGTGGCATATTACCATTAATTTGCATCAATTGGGCTGGCAACTAGGGAAGCAGGTTTTCT
CCATTGATGGCTGTGTCTAGTGGCTGAATGCTAAAAACAGTTACTAGTTTTGTTATCTAC
CAAGACCTCGTTAAGCATAGTTACAAGTTTTGTACTATTAGATCCAGACCTGAGTTATGT
TTTCTCTTTGGAAAATCTCTGAATCCTAATATTCATGTAGTCCAGAGCAAACAGTCGTGT
ATGGATCTTATCAGCCTTTGCTTCTTTATTTTTATAGGAACTGAGAGTGTGGAAGGAAAA
GAGGTGACAAGCGAGCAAATGATATCCGCATCTGACACTTCACTGCCTCTTGTGACAGTT
CCTGCTCCATCGAGCAAAGAGAATGGAGTCATGTGGGCNACTTCTCCTGATCGCCTGGAA
CTTCCCCCAAGAAAGAGTAAATGAGTATGATGGTCAATATGGAGAAATCTACATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAA
AAGAGTATTACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11