Sequences from confirmation amplicon


Confirmation sequences for line 505E11 and hit At1g56120 are shown.
Line ID 505E11
Locus At1g56120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20991908
Primers used for PCR EQ83 - 8474, show primer details
   
Sequence Name 64-K103246-0022-505-E11C-EQ83
GenBank Accession LN511178 [GenBank]
Sequencing primer gene specific primer, EQ83: 5'-GTATCCGACCTGTAAGTTCCACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K103246-0022-505-E11C-EQ83
TAGGAACAATCACCCCAACGCAAAAACTAAAATGTAAAGTAGTAAGTAGATTATAAAAAT
TATATCTTACGCGACTTCCAACTCCACAAAATTAGCAAATGATAAAGGTATTCCTCCGCT
GAGTCCAGAACTATCGATGTACCTGTAATAGATGTAAGAAATTTTTAAGAATAACAGATG
CACAAGACTTTTGTGGTCGACGAACACTAAAGAGTTTCAGACGTTAAGGTTTCATATCTN
AATTATAACTAGTCAATAACATAAGCACTTATGGATTTGACAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGA
   
Sequence Name 16-K103247-0022-505-E11C-8409
GenBank Accession LN493393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103247-0022-505-E11C-8409
TTACAATTGAATATATCCTGTCAATCCATAAGTGCTTATGTTATTGACTAGTTATAATTT
AGATATGAAACCTTAACGTCTGAAACTCTTTAGTGTTCGTCGACCACAAAAGTCTTGTGC
ATCTGTTATTCTTAAAAATTTCTTACATCTATTACAGGTACATCGATAGTTCTGGACTCA
GCGGAGGAATACCTTTATCATTTGCTAATTTTGTGGAGTTGGAAGTCGCGTAAGATATAA
TTTTTATAATCTACTTACTACTTTACATTTTAGTTTTTTGCGTTGGGGTGATTGTTTCCT
TAATGTTGTACCAGTTGGATTATGGATGTGGAACTTACAGGTCGGAT
Primers used for PCR KR69 - 3144, show primer details
   
Sequence Name 38-K106973-0022-505-E11C-3144
GenBank Accession LN502995 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K106973-0022-505-E11C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGCT
GTAGTTTTGTACAACTCCCAATCTCAGCTGGTAAGGAACCGGAAAAGTTATTTGAACTAA
TACCACTGCAGAAAATATATATAGCTATAAAGAACCATCAAATCCTCTCCATAAGCAGAG
TGAAGACATGTTCAAATATAGATAATGAGGAAGCAGCCAAAGCTTACAGTAATCTTAAAT
CCGTAAGCAAACCGATTTCCTTAGGAATAGGGCCAGACAACGCGTTGATTCCAAAAGTCC
TACATTTGAGCATGGGAGACAAACAATTGTTAATTGAACTTGTATAAAATTATCAAAAAA
GAAAAAGAAACAAGGTTTCAGAGCATTTTTACATCATC
   
Sequence Name 54-K106974-0022-505-E11C-KR69
GenBank Accession LN508493 [GenBank]
Sequencing primer gene specific primer, KR69: 5'-GATGTAAAAGCCTCTGAAACCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106974-0022-505-E11C-KR69
AGTTCATTAACAATTGTTTGTCTCCCATGCTCAAATGTAGGACTTTTGGAATCAACGCGT
TGTCTGGCCCTATTCCTAAGGAAATCGGTTTGCTTACGGATTTAAGATTACTGTAAGCTT
TGGCTGCTTCCTCATTATCTATATTTGAACATGTCTTCACTCTGCTTATGGAGAGGATTT
GATGGTTCTTTATAGCTATATATATTTTCTGCAGTGGTATTAGTTCAAATAACTTTTCCG
GTTCCTTACCAGCTGAGATTGGGAGTTGTACAAAACTACAGCAAACACTGATAGTTTAAA
CCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAA
CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACG
TCAGTGGAGATAT

Last Updated on 06.12.2018 16:11