Sequences from confirmation amplicon


Confirmation sequences for line 509C06 and hit At4g19210 are shown.
Line ID 509C06
Locus At4g19210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10503990
Primers used for PCR MD08 - 8474, show primer details
   
Sequence Name 29-K024507-022-509-C06C-8409
GenBank Accession LN499128 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K024507-022-509-C06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCATTGCTGATTCATGTA
GATCTGTCTGGTCCTTCTGTGTCGTCTGGTTTCAACAACCCGGCCTGCAAATATTACCAA
TATTACAACTTTACAGTACAGTACTTTCTAAAACTCTAACCACAAGAGAACATATCATGG
AAGAAGAGAATACCAGCATCCGAGTAAATGTTGTCTTCCCTGTACCAGTCTCCCCAAGCA
TTACAATAATCTGAGAGTCAGTGAATTCACCTTCCGACACTCTCAACCTGAAGTTTCCTT
GAGTTTTGGTCATCGTGGGGTATTTGTACCTAGCGTACGACTGTATCTCTTCAGCACTTT
CTTGTGGAGTCTCAGCAACCTTCAAACAAAAGAAA
   
Sequence Name 30-K024507-022-509-C06C-MD08
GenBank Accession LN499564 [GenBank]
Sequencing primer gene specific primer, MD08: 5'-TTTTTCTTTTGTTTGAAGGTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K024507-022-509-C06C-MD08
CACAAGAAAGTGCTGAAGAGATACAGTCGTACGCTAGGTACAAATACCCCACGATGACCA
AAACTCAAGGAAACTTCAGGTTGAGAGTGTCGGAAGGTGAATTCACTGACTCTCAGATTA
TTGTAATGCTTGGGGAGAATGGTACAGGGAAGACAACATTTATTCGGATGCTGGTATTCT
CTTCTTCCATGATATGTTCTCTTGTGGTTAGAGTTTTAGAAAGTACTGTACTGTAAAGTT
GTAATATTGGTAATATTTGCAGGCCGGGTTGTTGAAACCAGACGACACAGAAGGACCAGA
CAGATCTACATGAATCAGCAATGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGT

Last Updated on 06.12.2018 16:11