Sequences from confirmation amplicon


Confirmation sequences for line 510D03 and hit At1g21250 are shown.
Line ID 510D03
Locus At1g21250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7441365
Primers used for PCR CC80 - 8474, show primer details
   
Sequence Name 68-K106292-0022-510-D03C-8409
GenBank Accession LN512023 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106292-0022-510-D03C-8409
GGACTGAGCCATTTACAATTGAACATAGGTAACCTGTGTTGTTGGTATCAGCCTTGAAGC
ACCAAAGTCAGCTACTTTTGCAGTTAAGTTTACATCCAGAAGAATATTTGCAGTTTTGAT
ATCCCGATGGATGATTGGAATAGAAGCAGAGGAGTGAAGATATGCAAGAGTTCCAGCGAC
TTCTATTGCTATCTTCAGACGGTGTTCCCATGTAAGCGAAGAATCAATCATGGAACCATG
CAAGTGATCGAAAAGGGTGCCATTGGTGATGAACTCATAGACCAACAAGGGAACTTCAGT
CTCTAGACAGCAGCCCAAGAGCTTGACTACGTTCCTATGGTTGATTTGTGAAAGCACGAG
CACTTCATTGATGAACTGCTCTACTTGGCTACTGTCTCCAAGTCGGGCTTTCTTTATAGC
AACTATGGAGTTGTCCGGCAATATCCCTTTGTACACTGT
   
Sequence Name 68-K106293-0022-510-D03C-CC80
GenBank Accession LN512024 [GenBank]
Sequencing primer gene specific primer, CC80: 5'-AACAGTGTACAAAGGGATATTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106293-0022-510-D03C-CC80
TAGAGCAGTTCATCAATGAAGTGCTCGTGCTTTCACAAATCAACCATAGGAACGTAGTCA
AGCTCTTGGGCTGCTGTCTAGAGACTGAAGTTCCCTTGTTGGTCTATGAGTTCATCACCA
ATGGCACCCTTTTCGATCACTTGCATGGTTCCATGATTGATTCTTCGCTTACATGGGAAC
ACCGTCTGAAGATAGCAATAGAAGTCGCTGGAACTCTTGCATATCTTCACTCCTCTGCTT
CTATTCCAATCATCCATCGGGATATCAAAACTGCAAATATTCTTCTGGATGTAAACTTAA
CTGCAAAAGTAGCTGACTTTGGTGCTTCAAGGCTGATACCAACAACACAGGTTACCTATG
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCG

Last Updated on 06.12.2018 16:11