Sequences from confirmation amplicon


Confirmation sequences for line 510G03 and hit At3g56000 are shown.
Line ID 510G03
Locus At3g56000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20784246
Primers used for PCR IF51 - 8474, show primer details
   
Sequence Name 70-K101386-0022-510-G03C-8409
GenBank Accession LN512349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101386-0022-510-G03C-8409
GACCTTTTCAGAAATTGCAATGGAGATCATCAAAAACCAGGTAGACATAAACATAAATCC
CCAAATCTAATTCCTTTCGGAACACATATATAGTTCAATGACCTTTATCTTTTTTTGTGT
GACATGTTTGAAACAGAATGTGTCTTTATGGAAGAAGGTGTATTTGATATACAACTTCTT
CTTTTTGAGGAAGATTGTTGTACACATCTTCACATTCGTTTTCTACTGCGTGATTTTGCC
AGCTACCGTTATATTCCCGGAGATCGAAGTCCCTAAATGGACAACTATCTACATTCCAGC
TACCATCACTATCCTAAACGCTATTGCAACACCTAAATCATTTTACCTCATACTCTATTG
GATCTTATTTGAAAACGTAATGGCGATGCATAGGAGTATAGGAACTCTCATTGGATTGCT
AGAGACTAGTAGAGTTAAAGAATGGATTGTTACACAAAAACTAGGAGAGAGTAACAATCT
CAGGGAAAACCTCATTTTTCCTGATCACTA
   
Sequence Name 70-K101392-0022-510-G03C-IF51
GenBank Accession LN512350 [GenBank]
Sequencing primer gene specific primer, IF51: 5'-CAATGGACTGCAAGAAAAGATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101392-0022-510-G03C-IF51
AATATAGTATCCCATATGAAGAGATACATTCCAACCATTATCTCTCGCCATCGAAGTCTG
CACAAATTCGAAATCATATCATTGATTGATTAGCCATTTCGCACGATAAATCTATTCGGG
GAAAAAGTTCAAACCTCTCGGGGAAGCTATAGTGATCAGGAAAAATGAGGTTTTCCCTGA
GATTGTTACTCTCTCCTAGTTTTTGTGTAACAATCCATTCTTTAACTCTACTAGTCTCTA
GCAATCCAATGAGAGTTCCTATACTCCTATGCATCGCCATTACGTTTTCAAATAAGATCC
AATAGAGTATGAGGTAAAATGATTTAGGTGTTGCAATAGCGTTTAGGATAGTGATGGTAG
CTGGAATGTAGATAGTTGTCCATTTAGGGACTTCGATCTCCGGGAATATAACGGTAGCTG
GCAAAATCACGCAGTAGAAAACGAATGTGAAGATGTGTACAACAATCTTCCTCANAAAGA
AGAAGTTGTATATCANATACACCTTCTTCCATAAAGACACATTCTGTTTCAAACATGTCA
CACAAAAAAAGATAAAGGTCATTGAACTATATNTGTGTTCCGAAAGGAATTAGATNTGGG
GATTTATGTAATCTATCTACCTGGTTTTTGATGATCTCCATTGGCATTTTTCT

Last Updated on 06.12.2018 16:11