Sequences from confirmation amplicon


Confirmation sequences for line 510G08 and hit At1g60630 are shown.
Line ID 510G08
Locus At1g60630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22335082
Primers used for PCR EQ84 - 8474, show primer details
   
Sequence Name 39-K103252-0022-510-G08C-8409
GenBank Accession LN503319 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103252-0022-510-G08C-8409
TCGGACATGAGCCTTTACATTGAATATATCCCATGGGTGCGTGCGGTGAGAGAGGAAGAG
ACTGAAGTAAGTGAGGAGCTGAATGCTTCTGAGGAGAAACTTCAAGCTCTTTTGACCATC
GCGACAGCTTGCGTGGCTGTTAAGCCGGAAAACCGTCCTGCGATGAGAGAGGTATTGAAG
ATGGTGAAAGATGCAAGGGCTGAAGCAGCTCTTTTCTCATTTAACAGCAGTGATCATTCG
CCTGGGAGATGGTCGGATACGATTCAGAGCTTGCCAAGGGAGGATCATATGAGCATCTGA
TTCAAGTTGTTGTAGCTTTCTCTATTGTTTTGGTTTTTGAAGTAGCTAGGGGTTCAAAAT
ATAATATATGCTTCCTAAATTGGATAATACCCATAATTACATGATCACAATGGGTTGCTT
AACAAAAC
   
Sequence Name 87-K103253-0022-510-G08C-EQ84
GenBank Accession LN514757 [GenBank]
Sequencing primer gene specific primer, EQ84: 5'-ACTCAGTTTTGTTAAGCAACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K103253-0022-510-G08C-EQ84
TCCATTTAGGAAGCATATATTATATTTTGAACCCCTAGCTACTTCAAAACCAAAACAATA
GAGAAAGCTACAACAACTTGAATCAGATGCTCATATGATCCTCCCTTGGCAAGCTCTGAA
TCGTATCCGACCATCTCCCAGGCGAATGATCACTGCTGTTAAATGAGAAAAGAGCTGCTT
CAGCCCTTGCATCTTTCACCATCTTCAATACCTCTCTCATCGCAGGACGGTTTTCCGGCT
TAACAGCCACGCANGCTGTCGCGATGGTCAAAAGAGCTTGAAGTTTCTCCTCAGAAGCAT
TCAGCTCCTCACTTACTTCAGTCTCTTCCTCTCTCACCGCANGCACCCNTGTGGATATTT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGA
Primers used for PCR 01se - 8474, show primer details
   
Sequence Name 60-K118780-0022-510-G08C-01se
GenBank Accession LN510379 [GenBank]
Sequencing primer gene specific primer, 01se: 5'-ATTGCACACACACTCTTCTCTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118780-0022-510-G08C-01se
GTTGAAGTGTCTATTCGTAGAAGTAAGGTCTCTGGGAGTGGGAAGCCTCTTCACTGGACA
TCATGTTTGAAAATAGCAGAGGACTTGGCAATGGGTCTGGTCTACATTCATCAGAATCCT
GGCTTAACGCATGGGAACTTGAAATCATCTAATGTTTTGTTAGGTCCGGACTTCGAGTCC
TGTCTCACGGACTACGGTCTCAGCGATCTCCATGATCCTTACTCGATTGAAGACACAAGT
GCAGCTTCTCTCTTTTACAAAGCTCCTGAATGCAGAGACTTACGCAAGGCTTCAACACAA
CCAGCTGATGTTTATAGCTTTGGAGTCCTCCTTCTAGAGCTTCTAACAGGTAGAACTTCA
TTCAAGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTTATT
   
Sequence Name 20-K118779-0022-510-G08C-8409
GenBank Accession LN495431 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118779-0022-510-G08C-8409
AGCTCTAGAAGGAGGACTCCAAGCTATAAACATCAGCTGGTTGTGTTGAAGCCTTGCGTA
AGTCTCTGCATTCAGGAGCTTTGTAAAAGAGAGAAGCTGCACTTGTGTCTTCAATCGAGT
AAGGATCATGGAGATCGCTGAGACCGTAGTCCGTGAGACAGGACTCGAAGTCCGGACCTA
ACAAAACATTAGATGATTTCAAGTTCCCATGCGTTAAGCCAGGATTCTGATGAATGTAGA
CCAGACCCATTGCCAAGTCCTCTGCTATTTTCAAACATGATGTCCAGTGAAGAGGCTTCC
CACTCCCAGAGACCTTACTTCCTACGAATAAGACACATATCAAACAGATTAACACAAGTG
CTGAATCAGAGAAGAGTGGTTGTGCAAT

Last Updated on 06.12.2018 16:11