Sequences from confirmation amplicon


Confirmation sequences for line 512C03 and hit At4g24940 are shown.
Line ID 512C03
Locus At4g24940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12824973
Primers used for PCR SR06 - 8474, show primer details
   
Sequence Name 52-K103582-0022-512-C03C-SR06
GenBank Accession LN507788 [GenBank]
Sequencing primer gene specific primer, SR06: 5'-CATTCATATTTTGCTTTTTGCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103582-0022-512-C03C-SR06
AGCCTTGGCACGATTTGATATAGTGTGAGGTTGGTGTTTTGCCTTGCAGATTGACCAAAG
CTCATATCTTGGTATCTGGAATTAAGGGGACTGTTGCTGAGGTAGCTGTAACTCTATAAT
TCATGCTGAGATGTCATGCTTTTCAATTAGTTTAATCTAATGGTATCTTACTGCATTTCC
TGGCTTTGTTTCCATTTGGTGGGTTAGTTTTGCAAGAACATTGTGCTGGCAGGAGTTGGT
TCAGTGACTTTAATGGATGATCGTTTAGCAAACATGGAGGCCTTGAACGCTAACTTTTTG
ATTCCGCCTGATGAAAATGTTTACAGTGGCAAAACTGTGGCTGAGATTTGTAGTGATTCA
CTCAAGGATTTCAACCCTATGGTCCGTGTTTCCGTAGAGAAAGGTTTCCCTCTAATACCT
CTCCGTTAATGTTTTAAAAACATACCGACTATTATCTGATCAATTTTTCAAATCTTTCAG
GTGATTTGTCAATGCTTGGCACTGATTTTTTTGAGCAGTTTGATGTCGTAGTTATAGGCT
ATGGTTCTCGTGCTACCAAAGTATTTATACCACCTTTTACATATTTACCACTTGCTTTCA
TCTTTAGTTGCTCCATACATCTCTTCATGACGTTGTCTGTTTATTTTTTGCAGAAATATG
TCAATGAAAAATGTCGGAAGTTAAAGAAACGTGTCGCATTCTATACAGTCGATTGTAATC
ATGCANGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AA
   
Sequence Name 68-K103583-0022-512-C03C-8409
GenBank Accession LN511994 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K103583-0022-512-C03C-8409
TTGAATATATCCTGCATGATTACATCGACTGTATAGAATGCGACACGTTTCTTTAACTTC
CGACATTTTTCATTGACATATTTCTGCAAAAAATAAACAGACAACGTCATGAAGAGATGT
ATGGAGCAACTAAAGATGAAAGCAAGTGGTAAATATGTAAAAGGTGGTATAAATACTTTG
GTAGCACGAGAACCATAGCCTATAACTACGACATCAAACTGCTCAAAAAAATCAGTGCCA
AGCATTGACAAATCACCTGAAAGATTTGAAAAATTGATCAGATAATAGTCGGTATGTTTT
TAAAACATTAACGGAGAGGTATTAGAGGGAAACCTTTCTCTACGGAAACACGGACCATAG
GGTTGAAATCCTTGAGTGAATCACTACAAATCTCAGCCACAGTTTTGCCACTGTAAACAT
TTTCATCAGGCGGAATCAAAAAGTTAGCGTTCAAGGCCTCCATGTTTGCTAAACGATCAT
CCATTAAAGTCACTGAACCAACTCCTGCCAGCACAATGTTCTTGCAAAACTAACCCACCA
AATGGAAACAAAGCCAGGAAATGCAGTAAGATACCATTAGATTAAACTAATTGAAAAGCA
TGACATCTCAGCATGAATTATAGAGTTACAGCTACCTCAGCAACAGTCCCCTTAATTCCA
GATACCAAGATATGAGCTTTGGTCAATCTGCAAGGCAAAACACCAACCTCACACTATATC
ANATCGTGCCAAGCTTACAGAGTAATTACATGGTAGTGTAGTGTATCAATAAAATAAGCT
TCAGCAA
Primers used for PCR SR07 - 8474, show primer details
   
Sequence Name 34-K103731-0022-512-C03C-SR07
GenBank Accession LN501386 [GenBank]
Sequencing primer gene specific primer, SR07: 5'-GAGTTGCTTTTTGATTTCCAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103731-0022-512-C03C-SR07
GCCCTTCACTCTCTTCAAACACTTCTATTACTGAAAATTTCATGAGAATGCCAAAGATTA
GTGAAGAATTTTTGTTGTATGTATCTGCCTTGGGATCTCAATTTTCATTTGTGGAAAGTA
ATAAAGAAATCAGCTGTAGTAACTAGTAACTACCTCTCATAGCAAAGTAGAGCTTTGCTG
TTCTCCGAGGAATCGGTTTCCATGGTACCGATATAGCTTCCTGTGGCAATAAACAAATCT
AATTTATCACAAAATCCTATGCAGATGTAAGGATTATTAGTAGCGAGAACTAGTGTTAAA
TTGTACCTGAAAACTTGGAAAATTTAGTTCGCATTCCACCATCTCTTCAAGCTTTTTCTG
TGGAAAGAAAATTATCAGGAAAACAATACAAAGGACATTGTGCAAATTAGAAGTTGTTAA
TGAAGTTTAAATGTTCAATGGAATTATAGAAATTTAAAAATAAGCCAGATGAAATGTCAA
AACAGCACATAATCAACAGAAGAAATGCAACAACAAATCATGCAAGTACCTTTGTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCC
   
Sequence Name 50-K103732-0022-512-C03C-8409
GenBank Accession LN507141 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K103732-0022-512-C03C-8409
ACCATTTAACAAAGGTACTTGCATGATTTGTTGTTGCATTTCTTCTGTTGATTATGTGCT
GTTTTGACATTTCATCTGGCTTATTTTTAAATTTCTATAATTCCATTGAACATTTAAACT
TCATTAACAACTTCTAATTTGCACAATGTCCTTTGTATTGTTTTCCTGATAATTTTCTTT
CCACAGAAAAAGCTTGAAGAGATGGTGGAATGCGAACTAAATTTTCCAAGTTTTCAGGTA
CAATTTAACACTAGTTCTCGCTACTAATAATCCTTACATCTGCATAGGATTTTGTGATAA
ATTAGATTTGTTTATTGCCACAGGAAGCTATATCGGTACCATGGAAACCGATTCCTCGGA
GAACAGCAAAGCTCTACTTTGCTATGAGAGGTAGTTACTAGTTACTACAGCTGATTTCTT
TATTACTTTCCACAAATGAAAATTGAGATCCCAAGGCAGATACATACAACAAAAATTCTT
CACTAATCTTTGGCATTCTCATGAAATTTTCAGTAATAGAAGTGTTTGAAGAGAGTGAAG
GGCGTAAACATGGAGAATGTTCGCTACTCGATCTTGCCAGAGTCTTGGAAATCAAAAGGC
AAC

Last Updated on 06.12.2018 16:11