Sequences from confirmation amplicon


Confirmation sequences for line 512E05 and hit At5g10160 are shown.
Line ID 512E05
Locus At5g10160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3186185
Primers used for PCR 63ra - 8474, show primer details
   
Sequence Name 39-K115914-0022-512-E05C-63ra
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 63ra: 5'-CTCTTCATCAGTCCCTATCTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115914-0022-512-E05C-63ra
ATCAGCAATGGGAGATGAGGGGCAATATGGAGAAGAACAAAGAGGAATTACCAATTTTTT
TACGGATCAAAAATGTAGATGTCCGC
   
Sequence Name 13-K115913-0022-512-E05C-8409
GenBank Accession LN492190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115913-0022-512-E05C-8409
AAAGAACAAGGCTTTAATAAAATTATCACCCGTGAGGCAAAATCTCTTGTATCTTATTGA
TTGTCCATCACTTGTCGGAAACGCCTCATACCCTGACACAATTAAAGATTAACAATTTAT
AAAAGTTGAAACTTGATTTGAAAATCAGAGTGCAATAAGAGGGAAATTACTTAGTTCGAT
TGGGATCTCTTTCTCTGCGGTGCTCTTTGATTCATCAGTTGAGCATAAGACGAGTGAGCT
GGATCTTCGTTTGGGACGAAACGCGACGGAGCTGGATCTAAGCAACGGTGGAGATAGG

Last Updated on 06.12.2018 16:11