Sequences from confirmation amplicon


Confirmation sequences for line 512F02 and hit At5g36220 are shown.
Line ID 512F02
Locus At5g36220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14253906
Primers used for PCR 14kw - 8474, show primer details
   
Sequence Name 39-K113872-0022-512-F02C-14kw
GenBank Accession LN503424 [GenBank]
Sequencing primer gene specific primer, 14kw: 5'-ATGCAGATAATGGTAATAGCAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K113872-0022-512-F02C-14kw
TTTCCATTTGCTTAACCATCCTGACGTAATATCTAAAGCGAGAGACGAAATCGATAACCG
GGTTGGTTTAGACCGGCTTATCGAAGAAGCAGATCTTAGCGAGCTACCATATCTAAAGAA
CATTGTCCTTGAGACCCTTAGGCTACACCCAGCTACACCATTGCTAGTCCCACACATGGC
ATCAGAAGATTGCAAGATAGGATCCTACGATATGCCACGTGGTACAACGTTGTTGGTGAA
TGCATGGGCCATACATAGGGATCCAAACACGTGGGATGATCCGGATAGCTTTAAACCAGA
GCGGTTTGAGAAAGAAGAGGAAGCACAAAAGCTTCTGGCGTTTGGATTAGGTAGAAGAGC
GTGTCCTGGATCGGGTCTGGCCCAACGAATCGTGGGACTAGCTCTCGGGTCATTGATACA
ATGCTTTGAATGGGAGAGAGTTGGGAATGTGGAAGTGGATATGAAGGAAGGAGTAATTAC
CAACATGCAAGCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 13-K113871-0022-512-F02C-8409
GenBank Accession LN492122 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113871-0022-512-F02C-8409
TTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGTTGGTAATT
ACTCCTTCCTTCATATCCACTTCCACATTCCCAACTCTCTCCCATTCAAAGCATTGTATC
AATGACCCGAGAGCTAGTCCCACGATTCGTTGGGCCAGACCCGATCCAGGACACGCTCTT
CTACCTAATCCAAACGCCAGAAGCTTTTGTGCTTCCTCTTCTTTCTCAAACCGCTCTGGT
TTAAAGCTATCCGGATCATCCCACGTGTTTGGATCCCTATGTATGGCCCATGCATTCACC
AACAACGTTGTACCACGTGGCATATCGTAGGATCCTATCTTGCAATCTTCTGATGCCATG
TGTGGGACTAGCAATGGTGTAGCTGGGTGTAGCCTAAGGGTCTCAAGGACAATGTTCTTT
AGATATGGTAGCTCGCTAAGATCTGCTTCTTCGATAAGCCGGTCTAAACCAACCCGGTTA
TCGATTTCGTCTCTCGCTTTAGATATTACGTCAGGATGGTTAAGCAAATTGGAAAGTGCC
CATTCTAGAGTGACTGCTGATGTGTTAGTCCCTGCTATTACATAATTCTGCA

Last Updated on 06.12.2018 16:11