Sequences from confirmation amplicon


Confirmation sequences for line 512F06 and hit At4g19045 are shown.
Line ID 512F06
Locus At4g19045 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10438979
Primers used for PCR 46sm - 8474, show primer details
   
Sequence Name 32-K116518-0022-512-F06C-46sm
GenBank Accession LN500728 [GenBank]
Sequencing primer gene specific primer, 46sm: 5'-AAAGCGTGTTGTTTTGATCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116518-0022-512-F06C-46sm
ATGCACTTTAGGTAGTGGTAATCTTAGAGAAGCCGTCAAACTTCCTCCGGGAGAGGATTT
AAACGAATGGCTTGCAGTTAACAGTGAGCTTATAACTACACCATCACTTTTTCCTCATGG
TTTCACTCTGTTGATTAAGTTGTTGTTTTTCTTCAGCTGTGGATTTCTTCAATCAGGTGA
ATCTGTTATTTGGTACACTTACCGAGTTTTGCACCCCGGAGAACTGTTCTACAATGACTG
CTGGTCCCAAGTACAGATTTCTCTTCTTCATTATATAAGTAATTTTGGTGCCATTTACAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAACGGTTATTAT
   
Sequence Name 06-K116517-0022-512-F06C-8409
GenBank Accession LN488974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116517-0022-512-F06C-8409
AATTACTTATATAATGAAGAAGAGAAATCTGTACTTGGGACCAGCAGTCATTGTAGAACA
GTTCTCCGGGGTGCAAAACTCGGTAAGTGTACCAAATAACAGATTCACCTGATTGAAGAA
ATCCACAGCTGAAGAAAAACAACAACTTAATCAACAGAGTGAAACCATGAGGAAAAAGTG
ATGGTGTAGTTATAAGCTCACTGTTAACTGCAAGCCATTCGTTTAAATCCTCTCCCGGAG
GAAGTTTGACGGCTTCTCTAAGATTACCACTACCTAAAGTTGCATCTATATGCTTTCGAA
GCTCTGCTCCCTAAAAAGATCAAAACAACACGCTTTA

Last Updated on 06.12.2018 16:11