Sequences from confirmation amplicon


Confirmation sequences for line 513E07 and hit At3g61830 are shown.
Line ID 513E07
Locus At3g61830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22889098
Primers used for PCR MN64 - 8474, show primer details
   
Sequence Name 45-K103107-0022-513-E07C-MN64
GenBank Accession LN505493 [GenBank]
Sequencing primer gene specific primer, MN64: 5'-ACAAAAGCATCTCCAGCTACAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103107-0022-513-E07C-MN64
ACCCGTAGTAAGCAATGCCTCCGTGGTTGTCCTAAACCAAAAACGGCACTCAATGAGTCA
ATGTCTCATTTAGTCCAAACACAGTTTCACAAAAACACACCAAGATTAGAATCAATACCT
CTGAATATATGCTTAAACCTCCATTCAAAGCCATGAAGATCTCTAGTCACAAGTTCTTGA
GTAGGAGTAGCTTGTGTCATATCCTAAAAAGTGTAAAATTGGAGTGAGAATTCAAGCTAA
AGTTTCTTGAATTGAGAAGGTTTCTTTAAGAATATATTTACCAAAGAAGGCAAGCATTCA
GTGGCGTGTTTACGAAGAACAGAGAATCCACCATGAGTGCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAACATTATT
   
Sequence Name 45-K103108-0022-513-E07C-8409
GenBank Accession LN505494 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103108-0022-513-E07C-8409
TTACAATTGCACTCATGGTGGATTCTCTGTTCTTCGTAACACGCCACTGAATGCTTGCCT
TCTTTGGTAAATATATTCTTAAAGAAACCTTCTCAATTCAAGAAACTTTAGCTTGAATTC
TCACTCCAATTTTACACTTTTTAGGATATGACACAAGCTACTCCTACTCAAGAACTTGTG
ACTAGAGATCTTCATGGCTTTGAATGGAGGTTTAAGCATATATTCAGAGGTATTGATTCT
AATCTTGGTGTGTTTTTGTGAAACTGTGTTTGGACTAAATGAGACATTGACTCATTGAGT
GCCGTTTTTGGTTTAGGACAACCACGGAGGCATTTGCTTACTACGGGTTGGAGTACATTT
GTATCCTCGAAAAGACTTGTAGCTGGAGATGCTTTTG

Last Updated on 06.12.2018 16:11