Sequences from confirmation amplicon


Confirmation sequences for line 514B01 and hit At3g04605 are shown.
Line ID 514B01
Locus At3g04605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1247969
Primers used for PCR MG19 - 8474, show primer details
   
Sequence Name 10-K031691-022-514-B01C-MG19
GenBank Accession LN490534 [GenBank]
Sequencing primer gene specific primer, MG19: 5'-CACTCGAACACTTAGCTGTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K031691-022-514-B01C-MG19
CATTTCGAATGAAAGCAATCGCCATATCTCTCAGTGCTCTACGGCAACTCTTCACATCTG
GAAACTCTTGTCCAACGGTAAGTTGGTAAGTCATGTCAGCAGTGAGAGCTCGAGCTTGAA
CCGGAGTGCTAACCATAACCATTTCACCATGCTGATCAATTTCAAGTTCATGACTTGAGT
CAACGGCTAATTCCAGATTGTCATCTAGTTCATGGTTTTCCCCAACAACCAACTGATGAT
TATTATCTGACAATGAGAACTCATGGTTTTGCGATGAAAGAACCAGATCAGGATCAACAT
CGTCGTGTCCAGGCTTCTGATCAACCGAGATTTCATTCTCGTTCATATAATCGTGATGAT
GATGTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGAGGGGGG
   
Sequence Name 58-K031691-022-514-B01C-8409
GenBank Accession LN509608 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K031691-022-514-B01C-8409
CATTTACAATTGAATATATCCTGGGACATCATCATCACGATTATATGAACGAGAATGAAA
TCTCGGTTGATCAGAAGCCTGGACACGACGATGTTGATCCTGATCTGGTTCTTTCATCGC
AAAACCATGAGTTCTCATTGTCAGATAATAATCATCAGTTGGTTGTTGGGGAAAACCATG
AACTAGATGACAATCTGGAATTAGCCGTTGACTCAAGTCATGAACTTGAAATTGATCAGC
ATGGTGAAATGGTTATGGTTAGCACTCCGGTTCAAGCTCGAGCTCTCACTGCTGACATGA
CTTACCAACTTACCGTTGGACAAGAGTTTCCAGATGTGAAGAGTTGCCGTAGAGCACTGA
GAGATATGGCGATTGCTCTTCATTTCGAAATGCAGACTATAAAATCTGACAAAACTCGTT
TCACAGCTAAGTGTTCGAGTG

Last Updated on 06.12.2018 16:11