Sequences from confirmation amplicon


Confirmation sequences for line 514H06 and hit At1g52800 are shown.
Line ID 514H06
Locus At1g52800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19665014
Primers used for PCR CC82 - 8474, show primer details
   
Sequence Name 16-K106588-0022-514-H06C-8409
GenBank Accession LN493443 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106588-0022-514-H06C-8409
CGTTTTGGCGTTTGCGTTTTTGTAGCCTAAAGTTTTCAATTTCACCTACCTATGCAAGTA
TGTGAATTCTTGATTTCTTTATTGATGTGATTTTTTTCCCATGGTTAGGGTTGGAGCAAT
GACAGGATACGTTCTTGTGAACATAGAGTGACAATGGAAGGAGATAAGACGAGGTACACT
TTAGGTCTTTTCTCGTTTCTCACAGACTTGGTTTCCATTCCTGAGGAGCTTGTGGACGAC
AAACACCCACTTATGTATAAGCCATTCGATAACATCGCTCTCATA
   
Sequence Name 32-K106589-0022-514-H06C-CC82
GenBank Accession LN500597 [GenBank]
Sequencing primer gene specific primer, CC82: 5'-TTATGAGAGCGATGTTATCGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106589-0022-514-H06C-CC82
GCTCCTCAGGAATGGAAACCAAGTCTGTGAGAAACGAGAAAAGACCTAATGTGTACCTCG
TCTTATCTCCTTCCATTGTCACTCTATGCTCACAAGAACGTATCCTGTCATTGCTCCAAC
CCTAACCATGGGAAAAAAATCACATCAATAAAGAAATCAAGAATTCACATACTTGCATAG
TTAGGTGAAATTGAAAACTTTAGGCTACAAAAACTGCAAACGCCAAAACGACTTCATGTC
CTGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAACGTTATTA

Last Updated on 06.12.2018 16:11