Sequences from confirmation amplicon


Confirmation sequences for line 514H12 and hit At5g11910 are shown.
Line ID 514H12
Locus At5g11910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3836982
Primers used for PCR CC83 - 8474, show primer details
   
Sequence Name 73-K106292-0022-514-H12C-8409
GenBank Accession LN512903 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K106292-0022-514-H12C-8409
GCCATTTACAATTGAAGCCATTTACTTCATTCAAACTTTCCTGCAACGACCTCGAACAGT
ACCGGTAAATGCCAAACACCATGTAACCTGGGTTCTAACAATCATAAAACTAAAAAGATA
AAAGAATTACCCTTTCTGTTGCTAACATCAATGAATCCATTATCTTTGATTCTCTTGAAG
TAGTCCTTACCAAGCCGAAATTCGATTCCTCTGTCAAGAAAAAACCGCCCTGAGATATTC
ACAACAGTCTGCACATCATTGTACTTTGCTGCATACAAAAGCACCACATTTCCACCTGCA
CAAAATTCAATGACAACAACAGCAAAGCTCCAGTCTTCAGATATTCTTTCTTGAATTTTC
TGAAACATGAGATCCACATTAGTTTTACGTTCATATACCTTTACTATGTCCGATAATCGC
AGAAATCACACGGTTAACACCACGCAAGTGTTGCAAAACAGAGCGCAAATCTTCAACTTC
ACGGCGA
   
Sequence Name 73-K106293-0022-514-H12C-CC83
GenBank Accession LN512904 [GenBank]
Sequencing primer gene specific primer, CC83: 5'-ATTTCAGTATGGTAACTATCGCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K106293-0022-514-H12C-CC83
ACACATTGCGTGGTGTTACCGTGTGATTTCTGCGATTATCGGACATAGTAAAGGTATATG
AACGTAAAACTAATGTGGATCTCATGTTTCAGAAAATTCAAGAAAGAATATCTGAAGACT
GGAGCTTTGCTGTTGTTGTCATTGAATTTTGTGCAGGTGGAAATGTGGTGCTTTTGTATG
CAGCAAAGTACAATGATGTGCAGACTGTTGTGAATATCTCAGGGCGGTTTTTTCTTGACA
GAGGAATCGAATTTCGGCTTGGTAAGGACTACTTCAAGAGAATCAAAGATAATGGATTCA
TTGATGTTAGCAACAGAAAGGGTAATTCTTTTATCTTTTTAGTTTTATGATTGTTAGAAC
CAGGTTACATGGTGTTTGGCATTTACCGGTACTGTTCGAGGTCGTTGCAGGAAAGTTTGA
ATGAAGTAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGGTTTA

Last Updated on 06.12.2018 16:11